Expression profiling identifies a molecular signature of reactive astrocytes stimulated by cyclic AMP or proinflammatory cytokines

Girish C. Daginakatte, Adam Gadzinski, Ryan J. Emnett, Jennifer L. Stark, Ernesto R. Gonzales, Ping Yan, Jin Moo Lee, Anne H. Cross, David H. Gutmann

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Specialized glia, termed reactive astrocytes, accompany numerous pathologic conditions affecting the central nervous system, including stroke, multiple sclerosis, and neoplasia. To better define this important cell type, we employed high-density microarray gene expression profiling using two in vitro models of reactive gliosis (stimulation with dbcAMP or IL-1β/IFNγ). We identified 44 differentially expressed transcripts common to both in vitro models and demonstrated that a subset of these genes are also differentially expressed in response to experimental autoimmune encephalomyelitis and focal cerebral ischemia in vivo. Moreover, this pattern of differential gene expression is not observed in hyperproliferating or neoplastic glia.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalExperimental Neurology
Volume210
Issue number1
DOIs
StatePublished - Mar 2008

Keywords

  • Astrogliosis
  • EAE
  • GFAP
  • Glia
  • Glioma
  • Interferon-γ
  • Interleukin-1β
  • Microarray
  • Stroke

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