Expression of thin aggregative fimbriae promotes interaction of Salmonella typhimurium SR-11 with mouse small intestinal epithelial

S. Sukupolvi, R. G. Lorenz, Jeffrey Gordon, Z. Bian, John Pfeifer, S. J. Normark, M. Rhen

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The factors that mediate binding of Salmonella typhimurium to small intestinal epithelial cells have not been fully characterized. In this paper we demonstrate that elimination of production of thin aggregative fiber by a transposon insertion within the gene encoding the subunit protein of the fiber reduced binding of S. typhimurium SR-11 to a conditionally immortalized proximal small intestinal epithelial cell line established from transgenic mice. This binding defect could be overcome by transcomplementation with a wild-type allele. The conditionally immortalized cell line should prove useful in identifying the epithelial cell receptor for bacterial attachment since expression of its bacterial binding activity can be induced by manipulating the line's proliferative status.

Original languageEnglish
Pages (from-to)5320-5325
Number of pages6
JournalInfection and immunity
Volume65
Issue number12
StatePublished - Dec 12 1997

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