Expression of the starfish complement component C3 gene homolog under the influence of bacterial lipopolysaccharide

D. A. Mogilenko, I. V. Kudryavtsev, S. V. Orlov, A. D. Kharasova, A. V. Polevschikov

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A fragment of a homolog of the complement component C3 gene was cloned and sequenced from the starfish Asterias rubens. A phylogenetic analysis of the gene, termed ArC3-like, demonstrated its close similarity to the C3 gene homolog of deuterostome invertebrates. A high level of ArC3-like mRNA expression was observed in circulating cells (coelomocytes), a gut derivate (hepatopancreas), and the male gonad, but not in the stomach, female gonad, and rectal gland of A. rubens. ArC3-like gene expression was detected in all types of starfish coelomocytes, including lymphocyte-like cells and granular and nongranular amebocytes. Bacterial lipopolysaccharide (LPS) injected into the coelomic cavity of starfish increased the ArC3-like gene expression in coelomocytes and the hepatopancreas as compared to the control level (sterile sea water injection). The level of ArC3-like gene expression in coelomocytes in response to LPS reached its maximum 6 h after the injection and decreased to the baseline level 24 h after the injection. In the hepatopancreas, the level of ArC3-like gene expression reached its maximum 6-12 h after the LPS injection stimulation and remained high even 48 h after the injection. A long-term upregulation in response to LPS was demonstrated for the ArC3-like gene.

Original languageEnglish
Pages (from-to)67-76
Number of pages10
JournalMolecular Biology
Volume44
Issue number1
DOIs
StatePublished - Mar 2010

Keywords

  • Coelomocytes
  • Complement component C3
  • Echinoderms
  • Innate immunity
  • LPS
  • Starfish

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