Expression of the murine plasma cell nucleotide pyrophosphohydrolase PC-1 is shared by human liver, bone, and cartilage cells. Regulation of PC-1 expression in osteosarcoma cells by transforming growth factor-β

R. Huang, M. Rosenbach, R. Vaughn, D. Provvedini, N. Rebbe, S. Hickman, J. Goding, R. Terkeltaub

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

A bone and cartilage enzyme with both 5'-nucleotide phosphodiesterase I and nucleotide pyrophosphohydrolase (NTPPPH) activity modulates physiologic mineralization and pathologic chondrocalcinosis by generating inorganic pyrophosphate. We hypothesized that, as for alkaline phosphatase, expression of an NTPPPH gene can be shared by cells from bone, cartilage, and liver and by certain leukocytes. Recently, we demonstrated the hepatocyte and murine plasma cell membrane glycoprotein PC-1 to have both 5'-nucleotide phosphodiesterase I and NTPPPH activity. We detected polypeptides cross- reactive with PC-1 in human U20S osteosarcoma cells, articular chondrocytes, homogenized human knee cartilages, human knee synovial fluids, hepatoma cells, and murine plasmacytoma cells. Constitutive low abundance PC-1 mRNA expression was detected in U20S cells and chondrocytes by a nested RNA-PCR assay and by Northern blotting. TGFβ is known to substantially increase NTPPPH activity in primary osteoblast cultures. We demonstrated that TGFβ1 increased NTPPPH activity and the level of PC-1 mRNA and immunoprecipitable [35S]-methionine-labeled PC-1 polypeptides in U20S cells. The identification of PC-1 as an NTPPPH expressed in cells derived from bone and cartilage may prove useful in furthering the understanding of the role of NTPPPH in physiologic and pathologic mineralization.

Original languageEnglish
Pages (from-to)560-567
Number of pages8
JournalJournal of Clinical Investigation
Volume94
Issue number2
DOIs
StatePublished - Jan 1 1994

Keywords

  • PC-1
  • bone mineralization
  • chondrocalcinosis
  • nucleoside triphosphate pyrophosphohydrolase
  • transforming growth factor-β

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