TY - JOUR
T1 - Expression of the gene for the POU domain transcription factor Tst-1/Oct6 is regulated by an estrogen-dependent enhancer
AU - Renner, Karin
AU - Sock, Elisabeth
AU - Bermingham, John R.
AU - Wegner, Michael
N1 - Funding Information:
We thank Dr D. B. Evans and Ciba-Geigy AG, Basel, Switzerland for the generous gift of recombinant human estrogen receptor. In addition to basic support from the BMBF, this work was supported by a grant from the Deutsche Forschungsgemeinschaft to M.W. (We1326/5-1).
PY - 1996
Y1 - 1996
N2 - Expression of the POU domain protein Tst-1/Oct6 during development of glia and neurons is subject to a tight multifactorial control, Here we show that 17β-estradiol increases the level of endogenous Tst-1/Oct6 in glial cells. This effect was mediated at the level of gene expression by an enhancer present in the 5' flanking region of the mouse gene for Tst-1/Oct6, ~5 kb upstream of the transcriptional start site. The enhancer contained as the functional element a sequence motif that closely resembled a classical estrogen response element, It consisted of an imperfect palindrome with a spacing of 3 bp, and was bound in vitro by activated estrogen receptor. Furthermore, this element was able to confer estrogen responsiveness when introduced into a heterologous promoter. In the Tst-1/Oct6 gene enhancer, a TPA response element was found in close proximity to the estrogen receptor binding site. As a consequence, TPA and estrogen activated transcription of the Tst-1/Oct6 gene in a synergistic manner.
AB - Expression of the POU domain protein Tst-1/Oct6 during development of glia and neurons is subject to a tight multifactorial control, Here we show that 17β-estradiol increases the level of endogenous Tst-1/Oct6 in glial cells. This effect was mediated at the level of gene expression by an enhancer present in the 5' flanking region of the mouse gene for Tst-1/Oct6, ~5 kb upstream of the transcriptional start site. The enhancer contained as the functional element a sequence motif that closely resembled a classical estrogen response element, It consisted of an imperfect palindrome with a spacing of 3 bp, and was bound in vitro by activated estrogen receptor. Furthermore, this element was able to confer estrogen responsiveness when introduced into a heterologous promoter. In the Tst-1/Oct6 gene enhancer, a TPA response element was found in close proximity to the estrogen receptor binding site. As a consequence, TPA and estrogen activated transcription of the Tst-1/Oct6 gene in a synergistic manner.
UR - http://www.scopus.com/inward/record.url?scp=0029999573&partnerID=8YFLogxK
U2 - 10.1093/nar/24.22.4552
DO - 10.1093/nar/24.22.4552
M3 - Article
C2 - 8948648
AN - SCOPUS:0029999573
SN - 0305-1048
VL - 24
SP - 4552
EP - 4557
JO - Nucleic acids research
JF - Nucleic acids research
IS - 22
ER -