Expression of the G-CSF receptor on hematopoietic progenitor cells is not required for their mobilization by G-CSF

Fulu Liu, Jennifer Poursine-Laurent, Daniel C. Link

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

The mechanisms that regulate hematopoietic progenitor cell (HPC) mobilization from the bone marrow to blood have not yet been defined. HPC mobilization by granulocyte colony-stimulating factor (G-CSF), cyclophosphamide (CY), or interleukin-8 but not fit-3 ligand is markedly impaired in G-CSF receptor-deficient (G-CSFR-deficient) mice. G-CSFR is expressed on mature hematopoietic cells, HPCs, and stromal cells, which suggests that G-CSFR signals in one or more of these cell types was required for mobilization by these agents. To define the cell type(s) responsible for G-CSF-dependent mobilization, a series of chimeric mice were generated using bone marrow transplantation. Mobilization studies in these chimeras demonstrated that expression of the G-CSFR on transplantable hematopoietic cells but not stromal cells is required for CY- or G-CSF-induced mobilization. Moreover, in irradiated mice reconstituted with both wild type and G-CSFR-deficient bone marrow cells, treatment with CY or G-CSF resulted in the equal mobilization of both types of HPCs. This result held true for a broad spectrum of HPCs including colony-forming cells, CD34+ lineage- and Sca+ lineage- cells, and long-term culture initiating cells. Collectively, these data provide the first definitive evidence that expression of the G- CSFR on HPCs is not required for their mobilization by G-CSF and suggest, a model in which G-CSFR-dependent signals act in trans to mobilize HPCs from the bone marrow. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)3025-3031
Number of pages7
JournalBlood
Volume95
Issue number10
StatePublished - May 15 2000

Fingerprint Dive into the research topics of 'Expression of the G-CSF receptor on hematopoietic progenitor cells is not required for their mobilization by G-CSF'. Together they form a unique fingerprint.

  • Cite this