TY - JOUR
T1 - Expression of the cell surface proteoglycan glypican-5 is developmentally regulated in kidney, limb, and brain
AU - Saunders, Scott
AU - Paine-Saunders, Stephenie
AU - Lander, Arthur D.
N1 - Funding Information:
The authors thank Rob Chen, John Fesenko, Jon Ivins, and Asli Kumbasar for their helpful comments on the manuscript, and David Hobson and Vaishali Kulkarni for technical assistance. This work was supported by NIH Grant NS26862 to Arthur D. Lander. Scott Saunders is a Howard Hughes Medical Institute Physician Postdoctoral Fellow.
PY - 1997/10/1
Y1 - 1997/10/1
N2 - Heparan sulfate is ubiquitous at the cell surface, where it is expressed predominantly on proteoglycans of either the transmembrane syndecan family or the glycosylphosphatidylinositol (GPI)-anchored glypican family, and has been proposed to function as a 'coreceptor' for a number of 'heparin-binding' growth factors. Although little is known about functional differences between individual members of the glypican gene family, mutations in both the Drosophila gene dally and the human gene for glypican-3 strongly suggest that at least some glypicans do function in cellular growth control and morphogenesis. In particular, deletion of the human glypican-3 gene is responsible for Simpson-Golabi-Behmel syndrome, and its associated pie- and postnatal tissue overgrowth, increased risk of embryonal tumors during early childhood, and numerous visceral and skeletal anomalies. We have identified and characterized, by sequencing of EST clones and products of rapid amplification of cDNA ends (RACE), an mRNA that encodes a 572-amino-acid member of the glypican gene family (glypican-5) that is most related (50% amino acid similarity, 39% identity) to glypican-3. Glypican-5 mRNA is detected as a 3.9- and 4.4-kb transcript in adult and neonatal mouse brain total RNA, and in situ hybridization results localize transcript primarily to restricted regions of the developing central nervous system, limb, and kidney in patterns consistent with a role in the control of cell growth or differentiation. Interestingly, glypican-5 localizes to 13q31-32 of the human genome, deletions of which are associated with human 13q- syndrome, a developmental disorder with a pattern of defects that shows significant overlap with the pattern of glypican-5 expression.
AB - Heparan sulfate is ubiquitous at the cell surface, where it is expressed predominantly on proteoglycans of either the transmembrane syndecan family or the glycosylphosphatidylinositol (GPI)-anchored glypican family, and has been proposed to function as a 'coreceptor' for a number of 'heparin-binding' growth factors. Although little is known about functional differences between individual members of the glypican gene family, mutations in both the Drosophila gene dally and the human gene for glypican-3 strongly suggest that at least some glypicans do function in cellular growth control and morphogenesis. In particular, deletion of the human glypican-3 gene is responsible for Simpson-Golabi-Behmel syndrome, and its associated pie- and postnatal tissue overgrowth, increased risk of embryonal tumors during early childhood, and numerous visceral and skeletal anomalies. We have identified and characterized, by sequencing of EST clones and products of rapid amplification of cDNA ends (RACE), an mRNA that encodes a 572-amino-acid member of the glypican gene family (glypican-5) that is most related (50% amino acid similarity, 39% identity) to glypican-3. Glypican-5 mRNA is detected as a 3.9- and 4.4-kb transcript in adult and neonatal mouse brain total RNA, and in situ hybridization results localize transcript primarily to restricted regions of the developing central nervous system, limb, and kidney in patterns consistent with a role in the control of cell growth or differentiation. Interestingly, glypican-5 localizes to 13q31-32 of the human genome, deletions of which are associated with human 13q- syndrome, a developmental disorder with a pattern of defects that shows significant overlap with the pattern of glypican-5 expression.
UR - http://www.scopus.com/inward/record.url?scp=0031259923&partnerID=8YFLogxK
U2 - 10.1006/dbio.1997.8690
DO - 10.1006/dbio.1997.8690
M3 - Article
C2 - 9331333
AN - SCOPUS:0031259923
SN - 0012-1606
VL - 190
SP - 78
EP - 93
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -