Expression of phosphodiesterase 3 in rat submandibular gland cell lines

Taku Murata, Toshifumi Sugatani, Vincent C. Manganiello, Kasumi Shimizu, Toshiro Tagawa

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


A recent preliminary (unpublished) study showed that phosphodiesterase (PDE) 3A and 3B are expressed in rat submandibular glands. Here, PDE3 activity was detected in homogenates of rat submandibular gland acinar epithelial (SMIE) cells, but not rat A5 (epithelial duct) cells. Most of the PDE3 activity in SMIE cells was recovered in the particulate fraction. Only PDE3B mRNA was detected by reverse transcription-polymerase chain reaction in RNA from SMIE cells. The nucleotide sequence of the fragment was identical to the sequence of rat PDE3B. The PDE3 specific inhibitor, OPC3689 (10 and 50 μM), inhibited the growth of SMIE cells (19 and 63%), but not A5 cells. As the submandibular gland contains many types of cells, these results indicate that PDE3B may regulate a cAMP pool that is important in submandibular gland acinar epithelial cell function.

Original languageEnglish
Pages (from-to)453-457
Number of pages5
JournalArchives of Oral Biology
Issue number5
StatePublished - 2001
Externally publishedYes


  • Phosphodiesterase 3 or PDE 3
  • Phosphodiesterase 4 or PDE 4
  • Rat submandibular gland cell lines


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