TY - JOUR
T1 - Expression of nuclear factor one A and -B in the olfactory bulb
AU - Plachez, Céline
AU - Cato, Kathleen
AU - Mcleay, Robert C.
AU - Heng, Yee Hsieh Evelyn
AU - Bailey, Timothy L.
AU - Gronostasjki, Richard M.
AU - Richards, Linda J.
AU - Puche, Adam C.
AU - Piper, Michael
PY - 2012/10/1
Y1 - 2012/10/1
N2 - The nuclear factor one (NFI) family of transcription factors consists of four members in vertebrates, NFIA, NFIB, NFIC, and NFIX, which share a highly conserved N-terminal DNA-binding domain. NFI genes are widely expressed in the developing mouse brain, and mouse mutants lacking NFIA, NFIB, or NFIX exhibit developmental deficits in several areas, including the cortex, hippocampus, pons, and cerebellum. Here we analyzed the expression of NFIA and NFIB in the developing and adult olfactory bulb (OB), rostral migratory stream (RMS), and subventricular zone (SVZ). We found that NFIA and NFIB are expressed within these regions during embryonic and postnatal development and in the adult. Immunohistochemical analysis using cell-type-specific markers revealed that migrating neuroblasts in the adult brain express NFI transcription factors, as do astrocytes within the RMS and progenitor cells within the SVZ. Moreover, astrocytes within the OB express NFIA, whereas mitral cells within the OB express NFIB. Taken together these data show that NFIA and NFIB are expressed in both the developing and the adult OB and in the RMS and SVZ, indicative of a regulatory role for these transcription factors in the development of this facet of the olfactory system.
AB - The nuclear factor one (NFI) family of transcription factors consists of four members in vertebrates, NFIA, NFIB, NFIC, and NFIX, which share a highly conserved N-terminal DNA-binding domain. NFI genes are widely expressed in the developing mouse brain, and mouse mutants lacking NFIA, NFIB, or NFIX exhibit developmental deficits in several areas, including the cortex, hippocampus, pons, and cerebellum. Here we analyzed the expression of NFIA and NFIB in the developing and adult olfactory bulb (OB), rostral migratory stream (RMS), and subventricular zone (SVZ). We found that NFIA and NFIB are expressed within these regions during embryonic and postnatal development and in the adult. Immunohistochemical analysis using cell-type-specific markers revealed that migrating neuroblasts in the adult brain express NFI transcription factors, as do astrocytes within the RMS and progenitor cells within the SVZ. Moreover, astrocytes within the OB express NFIA, whereas mitral cells within the OB express NFIB. Taken together these data show that NFIA and NFIB are expressed in both the developing and the adult OB and in the RMS and SVZ, indicative of a regulatory role for these transcription factors in the development of this facet of the olfactory system.
KW - Neuroblast
KW - Olfactory bulb
KW - Rostral migratory stream
KW - Subventricular zone
UR - http://www.scopus.com/inward/record.url?scp=84865065456&partnerID=8YFLogxK
U2 - 10.1002/cne.23081
DO - 10.1002/cne.23081
M3 - Article
C2 - 22886731
AN - SCOPUS:84865065456
SN - 0021-9967
VL - 520
SP - 3135
EP - 3149
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 14
ER -