Expression of IgG Fc receptors in myeloid leukemic cell lines. Effect of colony-stimulating factors and cytokines

J. L. Liesveld, C. N. Abboud, R. J. Looney, D. H. Ryan, J. K. Brennan

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Three classes of FcR have been defined on human myeloid cells by their reactivity with mAb; FcRI (mAb 32); FcRII (mAb IV3); and FcRIII (mAb 3G8). We have quantitated the expression of each FcR on human myeloid leukemia cells and cell lines (KG-1, HL-60, U937, and K562). Detailed analysis of FcR surface expression is provided for the U937 cell line after exposure to CSF and cytokines. Increased expression of FcRI and FcRII occurred at 72 h in cells exposed to GCT or Mo cell line-conditioned medium as well as to medium from PHA-treated mononuclear cells. The augmentation of FcRII required protein synthesis and was diminished by a neutralizing antibody to granulocyte-macrophage CSF. We also show that fractions containing natural granulocyte CSF or granulocyte-macrophage CSF as well as r-granulocyte and r-granulocyte-macrophage CSF are capable of inducing FcRII on these cells, whereas other cytokines such IL-1 and IL-2, TNF-α, INF-γ and macrophage CSF failed to do so.

Original languageEnglish
Pages (from-to)1527-1533
Number of pages7
JournalJournal of Immunology
Volume140
Issue number5
StatePublished - 1988

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