@article{b21fcac057ba4f1da38f9547dd7e1510,
title = "Expression of hemolytically active murine fourth component of complement in transfected L cells",
abstract = "The S region of the murine major histocompatibility complex contains two related genes (C4-X and C4-Y), one of which encodes the fourth component of complement (C4). We have introduced cloned C4-X and C4-Y genes into L cells. C4-X transfectants contained no detectable C4-related RNA, whereas C4-Y transfectants contained large amounts of C4-related RNA. Culture supernatants of C4-Y transfectants contained processed C4 which was indistin-guishable from macrophage-derived C4. The L-cell-derived C4 restored hemolytic activity to C4-deficient serum, served as a substrate for C1s, and underwent autolytic cleavage suggesting the presence of an intramolecular thiolester bond. These experiments identify C4-Y as the functional C4 gene in H-2d mice and indicate that L cells can process pro-C4 correctly.",
author = "Chaplin, {David D.} and Robert Sackstein and Perlmutter, {David H.} and Weis, {John H.} and Kruse, {Torben A.} and John Coligan and Colten, {Harvey R.} and Seidman, {J. G.}",
note = "Funding Information: We thank Hakan Persson for discussion of methods of preparation of samples for radiosequencing. We are grateful to Arturo Ferreira for helpful discussion of the specificity of anti-C4 antisera. D. D. C. is a fellow of the Jane Coffin Childs Memorial Fund for Medical Research. This investigation has been aided by a grant from the Jane Coffin Chtlds Memorial Fund for Medical Research. D. H. P. IS supported by an American Liver Foundation Postdoctoral Fellowship Award. J. H. W. is the recipient of an American Cancer Society Fellowship. Additional support was from National Institutes of Health grants IF32AM07114 (D. H. P.), Al18436, Al19148 (J. G. S.), HD1746, Al20032 (H. R. C.). and American Cancer Society grant JFR66 (J. G. S.).",
year = "1984",
month = jun,
doi = "10.1016/0092-8674(84)90387-8",
language = "English",
volume = "37",
pages = "569--576",
journal = "Cell",
issn = "0092-8674",
number = "2",
}