Expression of feedback-resistant aspartate kinase gene in Corynebacterium crenatum

Zhi Zhao, Yang Jian Liu, Y. Wang, Ying Zi Zhang, Jiu Yuan Ding

Research output: Contribution to journalArticlepeer-review


The AEC-resistant aspartate kinase gene from C. crenatum CD945 was cloned into vector pJC1. Its expression was investigated both in the wild type C. crenatum AS1.542 and its mutant C. crenatum CD945. The result showed that C. crenatum AS1.542 harboring AK(fbr) gene could grow on the defined medium with the co-existence of 12 mg/mL both of AEC and L-threonine respectively. Overexpression of AK(fbr) gene in C. crenatum CD945 results in a 4-fold increase of specific enzyme activity than the parental strain. The amplification of the activity of aspartate kinase yields 22% increase of L-lysine production and 23% increase of L-lysine productivity without affecting the growth rate.

Original languageEnglish
Pages (from-to)530-533
Number of pages4
JournalWei sheng wu xue bao = Acta microbiologica Sinica
Issue number4
StatePublished - Aug 2005
Externally publishedYes


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