Expression of feedback-resistant aspartate kinase gene in Corynebacterium crenatum

Zhi Zhao; Yang Jian Liu; Y. Wang; Ying Zi Zhang; Jiu Yuan Ding

Expression of feedback-resistant aspartate kinase gene in Corynebacterium crenatum

Zhi Zhao, Yang Jian Liu, Y. Wang, Ying Zi Zhang, Jiu Yuan Ding

Research output: Contribution to journal › Article › peer-review

Abstract

The AEC-resistant aspartate kinase gene from C. crenatum CD945 was cloned into vector pJC1. Its expression was investigated both in the wild type C. crenatum AS1.542 and its mutant C. crenatum CD945. The result showed that C. crenatum AS1.542 harboring AK(fbr) gene could grow on the defined medium with the co-existence of 12 mg/mL both of AEC and L-threonine respectively. Overexpression of AK(fbr) gene in C. crenatum CD945 results in a 4-fold increase of specific enzyme activity than the parental strain. The amplification of the activity of aspartate kinase yields 22% increase of L-lysine production and 23% increase of L-lysine productivity without affecting the growth rate.

Original language	English
Pages (from-to)	530-533
Number of pages	4
Journal	Wei sheng wu xue bao = Acta microbiologica Sinica
Volume	45
Issue number	4
State	Published - Aug 2005
Externally published	Yes

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title = "Expression of feedback-resistant aspartate kinase gene in Corynebacterium crenatum",

abstract = "The AEC-resistant aspartate kinase gene from C. crenatum CD945 was cloned into vector pJC1. Its expression was investigated both in the wild type C. crenatum AS1.542 and its mutant C. crenatum CD945. The result showed that C. crenatum AS1.542 harboring AK(fbr) gene could grow on the defined medium with the co-existence of 12 mg/mL both of AEC and L-threonine respectively. Overexpression of AK(fbr) gene in C. crenatum CD945 results in a 4-fold increase of specific enzyme activity than the parental strain. The amplification of the activity of aspartate kinase yields 22% increase of L-lysine production and 23% increase of L-lysine productivity without affecting the growth rate.",

author = "Zhi Zhao and Liu, {Yang Jian} and Y. Wang and Zhang, {Ying Zi} and Ding, {Jiu Yuan}",

year = "2005",

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T1 - Expression of feedback-resistant aspartate kinase gene in Corynebacterium crenatum

AU - Zhao, Zhi

AU - Liu, Yang Jian

AU - Wang, Y.

AU - Zhang, Ying Zi

AU - Ding, Jiu Yuan

PY - 2005/8

Y1 - 2005/8

N2 - The AEC-resistant aspartate kinase gene from C. crenatum CD945 was cloned into vector pJC1. Its expression was investigated both in the wild type C. crenatum AS1.542 and its mutant C. crenatum CD945. The result showed that C. crenatum AS1.542 harboring AK(fbr) gene could grow on the defined medium with the co-existence of 12 mg/mL both of AEC and L-threonine respectively. Overexpression of AK(fbr) gene in C. crenatum CD945 results in a 4-fold increase of specific enzyme activity than the parental strain. The amplification of the activity of aspartate kinase yields 22% increase of L-lysine production and 23% increase of L-lysine productivity without affecting the growth rate.

AB - The AEC-resistant aspartate kinase gene from C. crenatum CD945 was cloned into vector pJC1. Its expression was investigated both in the wild type C. crenatum AS1.542 and its mutant C. crenatum CD945. The result showed that C. crenatum AS1.542 harboring AK(fbr) gene could grow on the defined medium with the co-existence of 12 mg/mL both of AEC and L-threonine respectively. Overexpression of AK(fbr) gene in C. crenatum CD945 results in a 4-fold increase of specific enzyme activity than the parental strain. The amplification of the activity of aspartate kinase yields 22% increase of L-lysine production and 23% increase of L-lysine productivity without affecting the growth rate.

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JO - Wei sheng wu xue bao = Acta microbiologica Sinica

JF - Wei sheng wu xue bao = Acta microbiologica Sinica

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ER -

Expression of feedback-resistant aspartate kinase gene in Corynebacterium crenatum

Abstract

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