TY - JOUR
T1 - Expression of factor V by resident macrophages boosts host defense in the peritoneal cavity
AU - Zhang, Nan
AU - Czepielewski, Rafael S.
AU - Jarjour, Nicholas N.
AU - Erlich, Emma C.
AU - Esaulova, Ekaterina
AU - Saunders, Brian T.
AU - Grover, Steven P.
AU - Cleuren, Audrey C.
AU - Broze, George J.
AU - Edelson, Brian T.
AU - Mackman, Nigel
AU - Zinselmeyer, Bernd H.
AU - Randolph, Gwendalyn J.
N1 - Funding Information:
This research was supported in large part by a National Institutes of Heath grant (5R37AI049653 to G.J. Randolph) and a National Institute of Diabetes and Digestive and Kidney Diseases pilot and feasibility grant (P30 DK052574 to B.H. Zinselmeyer), with additional support from the National Institutes of Heath (grants DP1DK109668 and R01HL118206 to G.J. Randolph), the American Heart Association (grant 16SDG30480008 to B.H. Zinselmeyer), National Institutes of Heath (grant R01AI113118 to B.T. Edelson), and a John C. Parker Endowed Professorship (to N. Mackman). The authors declare no competing financial interests.
Publisher Copyright:
© 2019 Zhang et al.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Macrophages resident in different organs express distinct genes, but understanding how this diversity fits into tissue-specific features is limited. Here, we show that selective expression of coagulation factor V (FV) by resident peritoneal macrophages in mice promotes bacterial clearance in the peritoneal cavity and serves to facilitate the well-known but poorly understood “macrophage disappearance reaction.” Intravital imaging revealed that resident macrophages were nonadherent in peritoneal fluid during homeostasis. Bacterial entry into the peritoneum acutely induced macrophage adherence and associated bacterial phagocytosis. However, optimal control of bacterial expansion in the peritoneum also required expression of FV by the macrophages to form local clots that effectively brought macrophages and bacteria in proximity and out of the fluid phase. Thus, acute cellular adhesion and resident macrophage–induced coagulation operate independently and cooperatively to meet the challenges of a unique, open tissue environment. These events collectively account for the macrophage disappearance reaction in the peritoneal cavity.
AB - Macrophages resident in different organs express distinct genes, but understanding how this diversity fits into tissue-specific features is limited. Here, we show that selective expression of coagulation factor V (FV) by resident peritoneal macrophages in mice promotes bacterial clearance in the peritoneal cavity and serves to facilitate the well-known but poorly understood “macrophage disappearance reaction.” Intravital imaging revealed that resident macrophages were nonadherent in peritoneal fluid during homeostasis. Bacterial entry into the peritoneum acutely induced macrophage adherence and associated bacterial phagocytosis. However, optimal control of bacterial expansion in the peritoneum also required expression of FV by the macrophages to form local clots that effectively brought macrophages and bacteria in proximity and out of the fluid phase. Thus, acute cellular adhesion and resident macrophage–induced coagulation operate independently and cooperatively to meet the challenges of a unique, open tissue environment. These events collectively account for the macrophage disappearance reaction in the peritoneal cavity.
UR - http://www.scopus.com/inward/record.url?scp=85067217186&partnerID=8YFLogxK
U2 - 10.1084/jem.20182024
DO - 10.1084/jem.20182024
M3 - Article
C2 - 31048328
AN - SCOPUS:85067217186
SN - 0022-1007
VL - 216
SP - 1291
EP - 1300
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 6
ER -