TY - JOUR
T1 - Expression of collagenase-3 (MMP-13) in human abdominal aortic aneurysms and vascular smooth muscle cells in culture
AU - Mao, Dongli
AU - Lee, Jason K.
AU - Vanvickle, Sarah J.
AU - Thompson, Robert W.
N1 - Funding Information:
1 Supported by NIH Grant R29 HL56701 from the National Heart, Lung, and Blood Institute and the Wylie Scholar Award from the Pacific Vascular Research Foundation, San Francisco, CA. 2 To whom correspondence should be addressed at Section of Vascular Surgery, Washington University School of Medicine, 9901 Wohl Hospital, 4960 Children’s Place, St. Louis, Missouri 63110. Fax: 314-747-3548. E-mail: [email protected].
PY - 1999/8/11
Y1 - 1999/8/11
N2 - Collagen degradation is important in the pathogenesis of abdominal aortic aneurysms (AAA) but the enzymes responsible are undefined. Collagenase-3 is a recently described matrix metalloproteinase (MMP-13) with limited tissue distribution and a highly regulated pattern of expression. Using reverse transcription-polymerase chain reaction and Southern blots, amplification products corresponding to MMP-13 were uniformly detected in samples of AAA and atherosclerotic aorta (ATH), but not in normal aortic controls. By densitometric analysis of blots normalized to β-actin, the expression of MMP-13 was 1.8-fold higher in AAA compared to ATH (P < 0.05). Immunoreactive MMP-13 was localized to medial smooth muscle cells (SMC) in AAA tissue and to human vascular SMC in culture, which also expressed MMP-13 mRNA. These findings indicate for the first time that SMC production of MMP-13 may contribute to the pathophysiologic progression of AAA.
AB - Collagen degradation is important in the pathogenesis of abdominal aortic aneurysms (AAA) but the enzymes responsible are undefined. Collagenase-3 is a recently described matrix metalloproteinase (MMP-13) with limited tissue distribution and a highly regulated pattern of expression. Using reverse transcription-polymerase chain reaction and Southern blots, amplification products corresponding to MMP-13 were uniformly detected in samples of AAA and atherosclerotic aorta (ATH), but not in normal aortic controls. By densitometric analysis of blots normalized to β-actin, the expression of MMP-13 was 1.8-fold higher in AAA compared to ATH (P < 0.05). Immunoreactive MMP-13 was localized to medial smooth muscle cells (SMC) in AAA tissue and to human vascular SMC in culture, which also expressed MMP-13 mRNA. These findings indicate for the first time that SMC production of MMP-13 may contribute to the pathophysiologic progression of AAA.
KW - Abdominal aortic aneurysm
KW - Collagenase-3
KW - Matrix metalloproteinases
KW - Polymerase chain reaction
KW - Vascular smooth muscle cells
UR - http://www.scopus.com/inward/record.url?scp=0033546724&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.1142
DO - 10.1006/bbrc.1999.1142
M3 - Article
C2 - 10441523
AN - SCOPUS:0033546724
SN - 0006-291X
VL - 261
SP - 904
EP - 910
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -