Expression of Cγ4 T cell receptors and lack of isotype exclusion by dendritic epidermal T cell lines

F. Koning, W. M. Yokoyama, W. L. Maloy, G. Stingl, T. J. McConnell, D. I. Cohen, E. M. Shevach, J. E. Coligan

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Although four murine Cγ gene segments (Cγ1, 2, 3, and 4) are known to exist, the large majority of expressed γ-chains have been shown to be of the Cγ1 isotype and no evidence exists for the expression of more than one receptor by γδ TCR-bearing cells. We investigated the nature of the TCR expressed on a number of murine dendritic epidermal T cell-derived cell lines by using both Northern blot and immunoprecipitation analyses. One of these CD3+ cell lines (T195) expresses Cγ4, Vγ1, and δ mRNA, and its CD3-associated TCR complex can be precipitated by both anti-Cγ4 and anti-δ sera, indicating that this receptor is a Cγ4/δ heterodimer. Furthermore, we show that two cell lines (Y245, Y93) express two distinct TCR γ-chains, one derived from the Cγ4 locus, whereas the second γ-chain is probably derived from the Cγ2 locus. Together with the previous demonstration of Cγ1/δTCR on a number of dendritic epidermal T cell lines (DETC), these results indicate that such DETC are capable of expressing a variety of γδ TCR and that, in some DETC, isotype exclusion of γ-chain expression does not occur.

Original languageEnglish
Pages (from-to)2057-2062
Number of pages6
JournalJournal of Immunology
Issue number6
StatePublished - Jan 1 1988


Dive into the research topics of 'Expression of Cγ4 T cell receptors and lack of isotype exclusion by dendritic epidermal T cell lines'. Together they form a unique fingerprint.

Cite this