TY - JOUR
T1 - Expression of a human α-1,3/4-fucosyltransferase in the pit cell lineage of FVB/N mouse stomach results in production of Leb-containing glycoconjugates
T2 - A potential transgenic mouse model for studying Helicobacter pylori infection
AU - Falk, Per G.
AU - Bry, Lynn
AU - Holgersson, Jan
AU - Gordon, Jeffrey I.
PY - 1995/2/28
Y1 - 1995/2/28
N2 - Helicobacter pylori is a human pathogen associated with the development of gastric and duodenal ulcers and gastric adenocarcinoma. To test the hypothesis that the human Lewisb blood group antigen (Leb) functions as a receptor for the bacteria's adhesins and mediates its attachment to gastric pit and surface mucous cells, a human α-1,3/4-fucosyltransferase was expressed in these cell lineages in FVB/N transgenic mice. The fucosyltransferase directed production of the Leb epitope without any apparent effect on the proliferation and differentiation programs of this lineage. Moreover, clinical isolates of H. pylori bound to these cells in transgenic mice but not in their normal littermates. Binding was blocked by pretreatment of the bacteria with soluble Leb. This mouse model could be useful for examining the molecular pathogenesis of diseases caused by H. pylori infection. Creating novel pathways for production of specific oligosaccharides in selected cell lineages of transgenic animals represents an approach for examining the role of complex carbohydrates in regulating cellular differentiation and host-microbe interactions.
AB - Helicobacter pylori is a human pathogen associated with the development of gastric and duodenal ulcers and gastric adenocarcinoma. To test the hypothesis that the human Lewisb blood group antigen (Leb) functions as a receptor for the bacteria's adhesins and mediates its attachment to gastric pit and surface mucous cells, a human α-1,3/4-fucosyltransferase was expressed in these cell lineages in FVB/N transgenic mice. The fucosyltransferase directed production of the Leb epitope without any apparent effect on the proliferation and differentiation programs of this lineage. Moreover, clinical isolates of H. pylori bound to these cells in transgenic mice but not in their normal littermates. Binding was blocked by pretreatment of the bacteria with soluble Leb. This mouse model could be useful for examining the molecular pathogenesis of diseases caused by H. pylori infection. Creating novel pathways for production of specific oligosaccharides in selected cell lineages of transgenic animals represents an approach for examining the role of complex carbohydrates in regulating cellular differentiation and host-microbe interactions.
UR - http://www.scopus.com/inward/record.url?scp=0028986626&partnerID=8YFLogxK
U2 - 10.1073/pnas.92.5.1515
DO - 10.1073/pnas.92.5.1515
M3 - Article
C2 - 7878011
AN - SCOPUS:0028986626
VL - 92
SP - 1515
EP - 1519
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 5
ER -