Expression of a gene encoding a unique protein-tyrosine kinase within specific fetal- and adult-derived hematopoietic lineages

Kyunghee Choi, Marion Kennedy, Gordon Keller

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

A gene encoding a unique protein-tyrosine kinase was isolated by PCR from undifferentiated embryonic stem (ES) cells. The gene, Emsk (embryonic stem cell kinase), is expressed in a number of different lymphoid and myeloid hematopoietic lineages and has been shown to be identical to the recently isolated focal adhesion-associated kinase gene (Fadk). Within the nonlymphoid lineages analyzed, Emsk/Fadk was found to be expressed in primitive and definitive erythroid cells but not in mast cells or macrophages. All CD5+ (B-1a) B cells tested, as well as freshly isolated conventional (B-2) B cells, expressed readily detectable levels of Emsk/Fadk. Within the T-cell lineage, Emsk/Fadk was expressed in Vγ5 γ/δ cells as well as in immature α/β cells found within the thymus. As the α/β T-cell population matures and exits the thymus, expression of Emsk/Fadk appears to be down regulated. The expression pattern outlined here suggests a role for Emsk/Fadk in multiple stages of hematopoietic development and raises the possibility that the kinase encoded by this gene has a broader spectrum of activities than was initially suggested.

Original languageEnglish
Pages (from-to)5747-5751
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number12
DOIs
StatePublished - Jun 15 1993

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