@article{5f3c19c47b684af086ba56039eab91be,
title = "Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein",
abstract = "Long chain fatty acids (LCFAs) are an important energy substrate used by cardiac myocytes and other cells, but the mechanism whereby these molecules cross the plasma membrane is poorly understood. We used an expression cloning strategy and a cDNA library from 3T3-L1 adipocytes to identify a cDNA that, when expressed in cultured cells, augments uptake of LCFAs. This cDNA encodes a novel 646 amino acid fatty acid transport protein (FATP) with six predicted membrane-spanning regions and that is integrally associated with membranes. Immunocytochemistry and subcellular fractionation of 3T3-L1 adipocytes show that FATP is localized to the plasma membrane. We propose that FATP is a plasma membrane transporter for LCFAs.",
author = "Schaffer, \{Jean E.\} and Lodish, \{Harvey F.\}",
note = "Funding Information: Correspondence should be addressed to H. F. L. We thank G. Baldini for the 3T3-Li adipocyte library, P. Brecher for the rat heart fatty acid-binding protein clone and monoclonal antiserum, G. Paradis and S. Conner for assistance with flow cytometry, M. Chafe1 for assistance with confocal microscopy, N. Cohen for technical assistance, W. Gilbert for help with sequence analysis, and S. Watowich, R. Wells, H. Wu, and D. Ory for assistance. We also thank P. Scherer, A. Mousta-kas, P. Murray, M. Krieger, and Ft. Rosenberg for critical review of this manuscript. J. E. S. is supported by a National Institutes of Health Physician Scientist Award (HL02696). Support for this work was also provided by a Program of Excellence in Molecular Biology grant from the National Heart, Lung, and Blood Institute (HL41484) and a grant from the Massachusetts affiliate of the American Heart Association.",
year = "1994",
month = nov,
day = "4",
doi = "10.1016/0092-8674(94)90252-6",
language = "English",
volume = "79",
pages = "427--436",
journal = "Cell",
issn = "0092-8674",
number = "3",
}