Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes

Grigory Maksaev, Elizabeth S. Haswell

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We have successfully expressed and characterized mechanosensitive channel of small conductance (MscS) from Escherichia coli in oocytes of the African clawed frog, Xenopus laevis. MscS expressed in oocytes has the same singlechannel conductance and voltage dependence as the channel in its native environment. Two hallmarks of MscS activity, the presence of conducting substates at high potentials and reversible adaptation to a sustained stimulus, are also exhibited by oocyte-expressed MscS. In addition to its ease of use, the oocyte system allows the user to work with relatively large patches, which could be an advantage for the visualization of membrane deformation. Furthermore, MscS can now be compared directly to its eukaryotic homologues or to other mechanosensitive channels that are not easily studied in E. coli.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalJournal of General Physiology
Volume138
Issue number6
DOIs
StatePublished - Dec 2011

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