Expressing and characterizing mechanosensitive channels in Xenopus oocytes

Grigory Maksaev, Elizabeth S. Haswell

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The oocytes of the African clawed frog (Xenopus laevis) comprise one of the most widely used membrane protein expression systems. While frequently used for studies of transporters and ion channels, the application of this system to the study of mechanosensitive ion channels has been overlooked, perhaps due to a relative abundance of native expression systems. Recent advances, however, have illustrated the advantages of the oocyte system for studying plant and bacterial mechanosensitive channels. Here we describe in detail the methods used for heterologous expression and characterization of bacterial and plant mechanosensitive channels in Xenopus oocytes.

Original languageEnglish
Pages (from-to)151-169
Number of pages19
JournalMethods in Molecular Biology
Volume1309
DOIs
StatePublished - 2015

Keywords

  • Heterologous expression
  • Mechanosensitive ion channel
  • Patch- clamp electrophysiology
  • Pressure-clamp
  • Xenopus laevis oocytes

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