Expressed protein ligation to study protein interactions: Semi-synthesis of the G-protein alpha subunit

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Abstract

Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.

Original languageEnglish
Pages (from-to)783-787
Number of pages5
JournalProtein and Peptide Letters
Volume12
Issue number8
DOIs
StatePublished - Nov 1 2005

Keywords

  • Expressed protein ligation
  • G protein
  • Intein
  • Rhodopsin
  • Transducin

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