Exonuclease V from Saccharomyces cerevisiae. A 5'→3'-deoxyribonuclease that produces dinucleotides in a sequential fashion

P. M.J. Burgers, G. A. Bauer, L. Tam

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11 Scopus citations

Abstract

A novel deoxyribonuclease, exonuclease V, has been purified approximately 30,000-fold from Saccharomyces cerevisiae. Exonuclease V is localized in the nucleus. The nuclease degrades single-stranded, but not double-stranded, DNA from the 5'-end. The products of exonuclease action are dinucleotides, except the 3'-terminal tri- and tetranucleotides which are not degraded. Studies with synthetic oligo- and polynucleotides with specified sequence elements showed that exonuclease V cleaves off dinucleotides as primary digestion products. Thus, the polymers (pT)9pA(pT)(n) and (pT)10pA(pT)(n) yielded pTpA and pApT as digestion products, respectively. Removal of the 5'-terminal phosphate from the DNA substrate results in reduced binding of the enzyme to the substrate. In addition, the initial hydrolytic cut by exonuclease V on the dephosphorylated substrate produces a mixture of dinucleoside monophosphates and trinucleoside diphosphates. The enzyme is processive in action.

Original languageEnglish
Pages (from-to)8099-8105
Number of pages7
JournalJournal of Biological Chemistry
Volume263
Issue number17
StatePublished - 1988

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