Exon-level expression profiling of ocular tissues

Alex H. Wagner; V. Nikhil Anand; Wan Heng Wang; Jon E. Chatterton; Duo Sun; Allan R. Shepard; Nasreen Jacobson; Iok Hou Pang; Adam P. DeLuca; Thomas L. Casavant; Todd E. Scheetz; Robert F. Mullins; Terry A. Braun; Abbot F. Clark

doi:10.1016/j.exer.2013.03.004

Exon-level expression profiling of ocular tissues

Alex H. Wagner, V. Nikhil Anand, Wan Heng Wang, Jon E. Chatterton, Duo Sun, Allan R. Shepard, Nasreen Jacobson, Iok Hou Pang, Adam P. DeLuca, Thomas L. Casavant, Todd E. Scheetz, Robert F. Mullins, Terry A. Braun, Abbot F. Clark

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.

Original language	English
Pages (from-to)	105-111
Number of pages	7
Journal	Experimental eye research
Volume	111
DOIs	https://doi.org/10.1016/j.exer.2013.03.004
State	Published - Jun 2013

Keywords

Database
Expression profile
Eye
Gene expression
Tissues

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10.1016/j.exer.2013.03.004

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title = "Exon-level expression profiling of ocular tissues",

abstract = "The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.",

keywords = "Database, Expression profile, Eye, Gene expression, Tissues",

author = "Wagner, {Alex H.} and Anand, {V. Nikhil} and Wang, {Wan Heng} and Chatterton, {Jon E.} and Duo Sun and Shepard, {Allan R.} and Nasreen Jacobson and Pang, {Iok Hou} and DeLuca, {Adam P.} and Casavant, {Thomas L.} and Scheetz, {Todd E.} and Mullins, {Robert F.} and Braun, {Terry A.} and Clark, {Abbot F.}",

year = "2013",

month = jun,

doi = "10.1016/j.exer.2013.03.004",

language = "English",

volume = "111",

pages = "105--111",

journal = "Experimental eye research",

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AU - Wagner, Alex H.

AU - Anand, V. Nikhil

AU - Wang, Wan Heng

AU - Chatterton, Jon E.

AU - Sun, Duo

AU - Shepard, Allan R.

AU - Jacobson, Nasreen

AU - Pang, Iok Hou

AU - DeLuca, Adam P.

AU - Casavant, Thomas L.

AU - Scheetz, Todd E.

AU - Mullins, Robert F.

AU - Braun, Terry A.

AU - Clark, Abbot F.

PY - 2013/6

Y1 - 2013/6

N2 - The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.

AB - The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.

KW - Database

KW - Expression profile

KW - Eye

KW - Gene expression

KW - Tissues

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SN - 0014-4835

VL - 111

SP - 105

EP - 111

JO - Experimental eye research

JF - Experimental eye research

ER -

Exon-level expression profiling of ocular tissues

Abstract

Keywords

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