Exon-level expression profiling of ocular tissues

Alex H. Wagner, V. Nikhil Anand, Wan Heng Wang, Jon E. Chatterton, Duo Sun, Allan R. Shepard, Nasreen Jacobson, Iok Hou Pang, Adam P. DeLuca, Thomas L. Casavant, Todd E. Scheetz, Robert F. Mullins, Terry A. Braun, Abbot F. Clark

    Research output: Contribution to journalArticlepeer-review

    89 Scopus citations

    Abstract

    The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.

    Original languageEnglish
    Pages (from-to)105-111
    Number of pages7
    JournalExperimental eye research
    Volume111
    DOIs
    StatePublished - Jun 2013

    Keywords

    • Database
    • Expression profile
    • Eye
    • Gene expression
    • Tissues

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