TY - JOUR
T1 - Excision and insertion of the conjugative transposon Tn916 involves a novel recombination mechanism
AU - Caparon, Michael G.
AU - Scott, June R.
N1 - Funding Information:
The expert technical assistance of Donald Stovall, Jeff Radcliff, and Joe Parsons is gratefully acknowledged. We thank Don Clewell for his interest, his helpful advice, and the kind gift of oligonucleotides. We thank Steve Warren for plorist6Xho. This work was supported in part by grant Al20723 from the Public Health Service to J. R. S. and in part by Biomedical Research Support Grant 507RR05364. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1989/12/22
Y1 - 1989/12/22
N2 - The covalently closed circular form of the conjugative transposon Tn916, which acts as an intermediate in transposition, is produced by a novel type of recombination. Excision of the element pairs noncomplementary base pairs, which flank the transposon in a heteroduplex, at the joint of a circular form. By a reversal of the excision process, the base pairs from the heteroduplex are inserted into the next target. We present a detailed molecular model for the movement of conjugative transposons that involves the initial formation of staggered nicks in the "coupling regions" that flank the inserted element. The different products of excision and insertion of Tn916 can be explained by this model.
AB - The covalently closed circular form of the conjugative transposon Tn916, which acts as an intermediate in transposition, is produced by a novel type of recombination. Excision of the element pairs noncomplementary base pairs, which flank the transposon in a heteroduplex, at the joint of a circular form. By a reversal of the excision process, the base pairs from the heteroduplex are inserted into the next target. We present a detailed molecular model for the movement of conjugative transposons that involves the initial formation of staggered nicks in the "coupling regions" that flank the inserted element. The different products of excision and insertion of Tn916 can be explained by this model.
UR - http://www.scopus.com/inward/record.url?scp=0024827010&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(89)90759-9
DO - 10.1016/0092-8674(89)90759-9
M3 - Article
C2 - 2557157
AN - SCOPUS:0024827010
SN - 0092-8674
VL - 59
SP - 1027
EP - 1034
JO - Cell
JF - Cell
IS - 6
ER -