Exchange of phospholipids between unilamellar vesicles of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine and plasma very low density lipoproteins

R. L. Jackson, D. Wilson, C. J. Glueck

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Purified phosphatidylcholine exchange protein from bovine liver was used to exchange [14C]dipalmitoyl phosphatidylcholine from sonicated vesicles to human plasma very low density lipoproteins (VLDL). The exchange of [14C]-dipalmitoyl phosphatidylcholine for VLDL phospholipids was temperature dependent and linear with respect to time and amount of exchange protein. In the absence of the exchange protein, less than 10% of the [14C]dipalmitoyl phosphatidylcholine was transferred. At an initial weight ratio of [14C]-dipalmitoyl phosphatidylcholine vesicles to VLDL phospholipid (1.2 mg) of 2.2, the exchange protein (14μg) replaced 55% of the VLDL phospholipids with [14C]dipalmitoyl phosphatidylcholine in 15 min; VLDL protein and cholesterol content were unaltered. From these studies we conclude that the exchange protein is a useful method to alter the phospholipid composition of VLDL under conditions such that there is minimal perturbation of the lipoprotein.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalBBA - Biomembranes
Volume557
Issue number1
DOIs
StatePublished - Oct 19 1979
Externally publishedYes

Keywords

  • (Human plasma)
  • Dipalmitoyl phosphatidylcholine
  • Phosphatidylcholine exchange protein
  • VLDL

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