@article{cb54806e337d49bd9664f9316b368597,
title = "Evolution of the hypoxia-sensitive cells involved in amniote respiratory reflexes",
abstract = "The evolutionary origins of the hypoxia-sensitive cells that trigger amniote respiratory reflexes – carotid body glomus cells, and {\textquoteleft}pulmonary neuroendocrine cells{\textquoteright} (PNECs) -are obscure. Homology has been proposed between glomus cells, which are neural crest-derived, and the hypoxia-sensitive {\textquoteleft}neuroepithelial cells{\textquoteright} (NECs) of fish gills, whose embryonic origin is unknown. NECs have also been likened to PNECs, which differentiate in situ within lung airway epithelia. Using genetic lineage-tracing and neural crest-deficient mutants in zebrafish, and physical fate-mapping in frog and lamprey, we find that NECs are not neural crest-derived, but endoderm-derived, like PNECs, whose endodermal origin we confirm. We discover neural crest-derived catecholaminergic cells associated with zebrafish pharyngeal arch blood vessels, and propose a new model for amniote hypoxia-sensitive cell evolution: endoderm-derived NECs were retained as PNECs, while the carotid body evolved via the aggregation of neural crest-derived catecholaminergic (chromaffin) cells already associated with blood vessels in anamniote pharyngeal arches.",
author = "Dorit Hockman and Burns, {Alan J.} and Gerhard Schlosser and Gates, {Keith P.} and Benjamin Jevans and Alessandro Mongera and Shannon Fisher and Gokhan Unlu and Knapik, {Ela W.} and Kaufman, {Charles K.} and Christian Mosimann and Zon, {Leonard I.} and Lancman, {Joseph J.} and Dong, {P. Duc S.} and Heiko Lickert and Tucker, {Abigail S.} and Baker, {Clare V.H.}",
note = "Funding Information: This work was funded by the Wellcome Trust (Ph.D. Studentship 086804/Z/08/Z to DH; Senior Investigator Award 102889/Z/13/Z to AST), the NIDCR/NIH (R21-DE021509 to SF; R01-DE018477 to EWK), the NIDDK/NIH (1DP2DK098092 to PDSD), NIH (R01-HL092217 to EWK), the Zebrafish Initiative of the Vanderbilt University Academic Venture Capital Fund (to EWK), the Vanderbilt Inter-national Scholar Program (to GU), the HFSP (Long-Term Fellowship to CM) and the Swiss National Science Foundation (Advanced Postdoctoral Fellowship and Professorship to CM). For financial sup-port, HL would like to thank the Helmholtz Society (Helmholtz Portfolio Theme {\textquoteright}Metabolic Dysfunc-tion and Common Disease{\textquoteright}), the Helmholtz Alliance (Imaging and Curing Environmental Metabolic Disease), and the German Center for Diabetes Research (DZD e.V.). Additional support for DH was provided by the Cambridge Trusts, the Cambridge Philosophical Society, the Oppenheimer Memorial Trust and Trinity College Oxford. DH thanks Tatjana Sauka-Spengler for her support and advice. AM thanks Christiane Nu¨ sslein-Volhard for her support and advice. Thanks to Colin Sharpe, Matt Guille and Alan Jafkins (University of Portsmouth, Portsmouth, UK) for access to transgenic Xenopus embryos and rearing of grafted embryos at the European Xenopus Research Centre (funded by the Wellcome Trust, the BBSRC and NC3Rs); to Christine Holt and Vasja Urban{\v c}i{\v c} (University of Cam-bridge, Cambridge, UK) for provision of wild-type Xenopus embryos; to Marianne Bronner (Califor-nia Institute of Technology, Pasadena, CA, USA) and David Parker (University of Cambridge, Cambridge, UK) for access to lampreys, and to Helen Sang and Adrian Sherman (Roslin Institute, Edinburgh, UK) for providing transgenic chicken eggs (Roslin Institute Transgenic Chicken Facility, Edinburgh, UK, funded by the Wellcome Trust and the BBSRC). Thanks to Perrine Barraud (University of Cambridge) for synthesizing the chicken Phox2b riboprobe, to Jana Koth (University of Oxford) for assistance in identifying zebrafish blood vessels, and to Giacomo Zaccone (University of Messina, Messina, Italy) for comments on an earlier version of the manuscript. Publisher Copyright: {\textcopyright} Hockman et al.",
year = "2017",
month = apr,
day = "7",
doi = "10.7554/eLife.21231",
language = "English",
volume = "6",
journal = "eLife",
issn = "2050-084X",
}