Evolution of cytomegalovirus-responsive T cell clonality following solid organ transplantation

Lauren E. Higdon, Steven Schaffert, Huang Huang, Maria E. Montez-Rath, Marc Lucia, Alokkumar Jha, Naresha Saligrama, Kenneth B. Margulies, Olivia M. Martinez, Mark M. Davis, Purvesh Khatri, Jonathan S. Maltzman

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

CMV infection is a significant complication after solid organ transplantation. We used single cell TCR ab sequencing to determine how memory inflation impacts clonality and diversity of the CMV-responsive CD8 and CD4 T cell repertoire in the first year after transplantation in human subjects. We observed CD8 T cell inflation but no changes in clonal diversity, indicating homeostatic stability in clones. In contrast, the CD4 repertoire was diverse and stable over time, with no evidence of CMV-responsive CD4 T cell expansion. We identified shared CDR3 TCR motifs among patients but no public CMV-specific TCRs. Temporal changes in clonality in response to transplantation and in the absence of detectable viral reactivation suggest changes in the repertoire immediately after transplantation followed by an expansion with stable clonal competition that may mediate protection.

Original languageEnglish
Pages (from-to)2077-2085
Number of pages9
JournalJournal of Immunology
Volume207
Issue number8
DOIs
StatePublished - Oct 15 2021

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