Evidence that the (6-4) photolyase mechanism can proceed through an oxetane intermediate

Saâdia Asgatay, Christian Petermann, Dominique Harakat, Dominique Guillaume, John Stephen Taylor, Pascale Clivio

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Using the analogue of TpT methylated at the 3′-end N3 position (Tpm3T), we demonstrate that when the oxetane/(6-4) pathway is precluded, water addition occurs at the 3′-end C6 position of the oxetane intermediate to provide its opening. Photoreversal of this (6-4) photoproduct C6 hydrate brings the first experimental evidence that the (6-4) photolyase repair can proceed through an oxetane intermediate.

Original languageEnglish
Pages (from-to)12618-12619
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number38
DOIs
StatePublished - Sep 24 2008

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