TY - JOUR
T1 - Evidence for Z-form RNA by vacuum UV circular dichroism
AU - Riazance, Jeannine H.
AU - Baase, Walter A.
AU - Johnson, W. Curtis
AU - Hall, Kathleen
AU - Cruz, Phillip
AU - Tinoco, Ignacio
N1 - Funding Information:
ACKNOWLEDGMENT This work was supported in part by N1H grant GN 21479 and NSF grant DMB 8415499 at Corvallls, NSF grant PCM 8104339 at Eugene, and NTH grant GM 10840 and the U.S. Department of Energy, Office of Energy Research Contract 82ER60090 at Berkeley.
PY - 1985/7/11
Y1 - 1985/7/11
N2 - Circular dichroism (CD) spectra in the vacuum UV region for different conformations of poly d(G-C).poly d(G-C) and poly r(G-C)·poly r(G-C) are very characteristic. The CD of the RNA in the A-form (6 N NaClO4 and 22°C) is very similar to that of the DNA in 80% alcohol where it is believed to be in the A-form. With the exception of the longest wavelength transition, the CD of the RNA in 6 N NaClO4 at 46°C is similar.ar to the CD of the DNA under conditions where it is believed to be in the Z-form (2 M NaClO4 This substantiates that poly r(G-C)·poly r(G-C) assumes a left-handed Z-conformation in 6 M NaClO4 above 35°C. CD spectra for the left-handed Z-forms of both the RNA and DNA are characterized by an intense negative peak at 190-195 nm, a crossover at about 184 nm, and an intense positive peak below 180 nm. The right-handed A- and B-forms of RNA and DNA all have an intense positive peak in their CD spectra near 186 nm. The large difference in CD in the range 185-195 rim for right- and left-handed conformations of nucleic acids can be used to identify the sense of helix winding.
AB - Circular dichroism (CD) spectra in the vacuum UV region for different conformations of poly d(G-C).poly d(G-C) and poly r(G-C)·poly r(G-C) are very characteristic. The CD of the RNA in the A-form (6 N NaClO4 and 22°C) is very similar to that of the DNA in 80% alcohol where it is believed to be in the A-form. With the exception of the longest wavelength transition, the CD of the RNA in 6 N NaClO4 at 46°C is similar.ar to the CD of the DNA under conditions where it is believed to be in the Z-form (2 M NaClO4 This substantiates that poly r(G-C)·poly r(G-C) assumes a left-handed Z-conformation in 6 M NaClO4 above 35°C. CD spectra for the left-handed Z-forms of both the RNA and DNA are characterized by an intense negative peak at 190-195 nm, a crossover at about 184 nm, and an intense positive peak below 180 nm. The right-handed A- and B-forms of RNA and DNA all have an intense positive peak in their CD spectra near 186 nm. The large difference in CD in the range 185-195 rim for right- and left-handed conformations of nucleic acids can be used to identify the sense of helix winding.
UR - http://www.scopus.com/inward/record.url?scp=0022423463&partnerID=8YFLogxK
U2 - 10.1093/nar/13.13.4983
DO - 10.1093/nar/13.13.4983
M3 - Article
C2 - 2410859
AN - SCOPUS:0022423463
VL - 13
SP - 4983
EP - 4989
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 13
ER -