Double-negative (CD4-/CD8-) T cells expressing the α/β transgenic TCR from the 2C cell line (anti-H-2L(d)) were examined in the periphery of animals whose MHC type produces positive, negative, or no selection for differentiation of the TCR on single positive (CD8+) cells. Regardless of the selection haplotype the CD4-/CD8- cells are capable of activation by anticlonotypic mAb indicating that negative selection does not inactivate the 'forbidden' TCR. Rather, the lack of response to H-2L(d) in the negative haplotype is likely the absence of CD8 required to produce a functional response to H-2L(d). The similarity of surface phenotype and functional activity of these CD4-/CD8- cells maturing in different haplotypes suggests they may arise by an alternative lineage which, unlike the dominant TCR α/β pathway, does not require coexpression of CD4/CD8.
|Number of pages||8|
|Journal||Journal of Immunology|
|State||Published - 1990|