Evidence for CD8-independent T cell maturation in transgenic mice

J. H. Russell, P. Meleedy-Rey, D. E. McCulley, W. C. Sha, C. A. Nelson, D. Y. Loh

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55 Scopus citations


Double-negative (CD4-/CD8-) T cells expressing the α/β transgenic TCR from the 2C cell line (anti-H-2L(d)) were examined in the periphery of animals whose MHC type produces positive, negative, or no selection for differentiation of the TCR on single positive (CD8+) cells. Regardless of the selection haplotype the CD4-/CD8- cells are capable of activation by anticlonotypic mAb indicating that negative selection does not inactivate the 'forbidden' TCR. Rather, the lack of response to H-2L(d) in the negative haplotype is likely the absence of CD8 required to produce a functional response to H-2L(d). The similarity of surface phenotype and functional activity of these CD4-/CD8- cells maturing in different haplotypes suggests they may arise by an alternative lineage which, unlike the dominant TCR α/β pathway, does not require coexpression of CD4/CD8.

Original languageEnglish
Pages (from-to)3318-3325
Number of pages8
JournalJournal of Immunology
Issue number9
StatePublished - 1990


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