Evidence for a colonic paf receptor

Walter E. Longo, Jeffrey D. Carter, Bhugol Chandel, Michael Niehoff, John Standeven, Yashwant Deshpande, Anthony M. Vernava, Gregory Polites, Anil D. Kulkarni, Donald L. Kaminski

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


We sought to determine the effect of exogenously administered platelet-activating factor (PAF) on eicosanoid release from the left colon of the rabbit. Using an isolated buffer-perfused rabbit left colon preparation, 1.0- or 5.0-μg doses of PAF were infused into the inferior mesenteric artery. Effluents from the inferior mesenteric vein and colonic lumen were collected and the concentrations of the eicosanoids, prostaglandin E, 6-ketoprostaglandin F, thromboxane B2, and leukotriene B4 (LTB4), were measured by ELISA. During PAF infusion there was a significant increase of all prostanoids, but not LTB4 into the venous effluent and colonic luminal perfusate when compared to control experiments. Additional studies were performed by pretreating the colons with the PAF antagonists WEB-2170 or alprazolam prior to PAF infusion. Both venous and luminal effluent prostanoid release was effectively blocked by WEB-2170, but not by alprazolam. Colons pretreated with WEB-2170 prior to PAF had markedly diminished tissue injury when compared to colons treated with PAF alone. Inhibition of PAF-stimulated prostanoid release by WEB-2170 suggests that a PAF-sensitive receptor is present in rabbit colonic tissue which may induce eicosanoid-mediated tissue injury.

Original languageEnglish
Pages (from-to)12-18
Number of pages7
JournalJournal of Surgical Research
Issue number1
StatePublished - Jan 1995


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