TY - JOUR
T1 - Evaluation of the pediatric Septi-Chek blood culture system
AU - Welby, Patricia L.
AU - Zusag, Theresa M.
AU - Orlando, Mary Fran
AU - Storch, Gregory A.
N1 - Funding Information:
This work was supported in part by Roche Diagnostics Systems, Nutley, New Jersey. We are grateful for the cooperation of the staff of the Microbiology Laboratory of the Washington University Department of Pediatrics.
PY - 1992
Y1 - 1992
N2 - The pediatric Septi-Chek blood culture system is a biphasic system that uses a 20-ml bottle of brain-heart infusion broth. We compared this system with a supplemented peptone broth tube (Vacutainer) for the recovery of aerobic organisms from blood cultures obtained from patients in a pediatric hospital. Blood specimens for culture were drawn into transport tubes containing sodium polyanetholsulfonate (SPS), and equal volumes were allocated into a Septi-Chek bottle and a Vacutainer tube which was vented for the first day of incubation. A total of 4828 blood culture sets was included, from which 243 probable pathogens were recovered, including 211 in the Septi-Chek system and 204 from the Vacutainer tube. There were no significant differences in the recovery of individual pathogens. The mean time to initial detection of pathogens was comparable in the two systems, but the mean time to growth on solid media for pathogens recovered in both systems was shorter with Septi-Chek (37.2 hr compared with 45.5 hr, p < 0.001). The pediatric Septi-Chek system is comparable with a vented Vacutainer tube for the recovery of aerobic pathogens, and its use facilitates the early identification and susceptibility testing of bloodstream pathogens.
AB - The pediatric Septi-Chek blood culture system is a biphasic system that uses a 20-ml bottle of brain-heart infusion broth. We compared this system with a supplemented peptone broth tube (Vacutainer) for the recovery of aerobic organisms from blood cultures obtained from patients in a pediatric hospital. Blood specimens for culture were drawn into transport tubes containing sodium polyanetholsulfonate (SPS), and equal volumes were allocated into a Septi-Chek bottle and a Vacutainer tube which was vented for the first day of incubation. A total of 4828 blood culture sets was included, from which 243 probable pathogens were recovered, including 211 in the Septi-Chek system and 204 from the Vacutainer tube. There were no significant differences in the recovery of individual pathogens. The mean time to initial detection of pathogens was comparable in the two systems, but the mean time to growth on solid media for pathogens recovered in both systems was shorter with Septi-Chek (37.2 hr compared with 45.5 hr, p < 0.001). The pediatric Septi-Chek system is comparable with a vented Vacutainer tube for the recovery of aerobic pathogens, and its use facilitates the early identification and susceptibility testing of bloodstream pathogens.
UR - http://www.scopus.com/inward/record.url?scp=0026754129&partnerID=8YFLogxK
U2 - 10.1016/0732-8893(92)90015-L
DO - 10.1016/0732-8893(92)90015-L
M3 - Article
C2 - 1611846
AN - SCOPUS:0026754129
SN - 0732-8893
VL - 15
SP - 301
EP - 306
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -