@article{7ebc2397b1b64938b7686090b92ff2a7,
title = "Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence",
abstract = "Previous studies have demonstrated that a high-protein diet leads to increased atherosclerosis in mice, and that this adverse effect is caused by activation of macrophage mTORC1 signaling. Here, we provide a detailed protocol for the evaluation of diet-induced mTORC1 signaling in plaque macrophages in atherosclerosis-prone apolipoprotein E (ApoE) knockout (KO) mice. This protocol includes flow cytometry and immunofluorescence analysis of atherosclerotic macrophages that can be used to study the atherogenic potential of a variety of mTORC1 modulators. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2020).",
keywords = "Cell isolation, Flow cytometry/Mass cytometry, Immunology",
author = "Xiangyu Zhang and Jeremiah Stitham and Astrid Rodriguez-Velez and Jeong, {Se Jin} and Arick Park and Yeh, {Yu Sheng} and Divya Kapoor and Bettina Mittendorfer and Babak Razani",
note = "Funding Information: This work was supported by NIH grants R01 HL125838 , R01 DK121560 , P30 DK056341 (Washington University Nutrition and Obesity Research Center), and T32 HL134635 and grants from the American Heart Association (# 897628 ), the Veterans{\textquoteright} Administration ( I01 BX003415 award), and the Longer Life Foundation . Cell sorting and flow cytometry data acquisition and analysis provided by the Flow Cytometry Core facility, Department of Pathology and Immunology, Washington University School of Medicine. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = dec,
day = "16",
doi = "10.1016/j.xpro.2022.101665",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
number = "4",
}