Evaluation of isotope discrimination in 13C-based metabolic flux analysis

Xueyang Feng; Yinjie J. Tang

doi:10.1016/j.ab.2011.06.022

Evaluation of isotope discrimination in ¹³C-based metabolic flux analysis

Xueyang Feng
, Yinjie J. Tang

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

In a ¹³C experiment for metabolic flux analysis (¹³C MFA), we examined isotope discrimination by measuring the labeling of glucose, amino acids, and hexose monophosphates via mass spectrometry. When Escherichia coli grew in a mix of 20% fully labeled and 80% naturally labeled glucose medium, the cell metabolism favored light isotopes and the measured isotopic ratios (δ¹³C) were in the range of -35 to -92. Glucose transporters might play an important role in such isotopic fractionation. Flux analysis showed that both isotopic discrimination and isotopic impurities in labeled substrates could affect the solution of ¹³C MFA.

Original language	English
Pages (from-to)	295-297
Number of pages	3
Journal	Analytical Biochemistry
Volume	417
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2011.06.022
State	Published - Oct 15 2011

Keywords

Amino acid
Glucose transporter
Isotopic impurity
Mass spectrometer

Access to Document

10.1016/j.ab.2011.06.022

Cite this

@article{28586250129942f286aa988aa6d0cdf0,

title = "Evaluation of isotope discrimination in 13C-based metabolic flux analysis",

abstract = "In a 13C experiment for metabolic flux analysis (13C MFA), we examined isotope discrimination by measuring the labeling of glucose, amino acids, and hexose monophosphates via mass spectrometry. When Escherichia coli grew in a mix of 20\% fully labeled and 80\% naturally labeled glucose medium, the cell metabolism favored light isotopes and the measured isotopic ratios (δ13C) were in the range of -35 to -92. Glucose transporters might play an important role in such isotopic fractionation. Flux analysis showed that both isotopic discrimination and isotopic impurities in labeled substrates could affect the solution of 13C MFA.",

keywords = "Amino acid, Glucose transporter, Isotopic impurity, Mass spectrometer",

author = "Xueyang Feng and Tang, \{Yinjie J.\}",

note = "Funding Information: We appreciate the help of Baichen Zhang and Leslie Hicks at the Danforth Plant Science Center in providing LC–MS service. We are grateful to David Fike at Washington University for help with isotope ratio mass spectrometry measurement. We thank James Ballard in the Engineering Communication Center of Washington University for editing the manuscript. The study was supported by the National Science Foundation (NSF, MCB0954016 ). ",

year = "2011",

month = oct,

day = "15",

doi = "10.1016/j.ab.2011.06.022",

language = "English",

volume = "417",

pages = "295--297",

journal = "Analytical Biochemistry",

issn = "0003-2697",

number = "2",

}

TY - JOUR

T1 - Evaluation of isotope discrimination in 13C-based metabolic flux analysis

AU - Feng, Xueyang

AU - Tang, Yinjie J.

N1 - Funding Information: We appreciate the help of Baichen Zhang and Leslie Hicks at the Danforth Plant Science Center in providing LC–MS service. We are grateful to David Fike at Washington University for help with isotope ratio mass spectrometry measurement. We thank James Ballard in the Engineering Communication Center of Washington University for editing the manuscript. The study was supported by the National Science Foundation (NSF, MCB0954016 ).

PY - 2011/10/15

Y1 - 2011/10/15

N2 - In a 13C experiment for metabolic flux analysis (13C MFA), we examined isotope discrimination by measuring the labeling of glucose, amino acids, and hexose monophosphates via mass spectrometry. When Escherichia coli grew in a mix of 20% fully labeled and 80% naturally labeled glucose medium, the cell metabolism favored light isotopes and the measured isotopic ratios (δ13C) were in the range of -35 to -92. Glucose transporters might play an important role in such isotopic fractionation. Flux analysis showed that both isotopic discrimination and isotopic impurities in labeled substrates could affect the solution of 13C MFA.

AB - In a 13C experiment for metabolic flux analysis (13C MFA), we examined isotope discrimination by measuring the labeling of glucose, amino acids, and hexose monophosphates via mass spectrometry. When Escherichia coli grew in a mix of 20% fully labeled and 80% naturally labeled glucose medium, the cell metabolism favored light isotopes and the measured isotopic ratios (δ13C) were in the range of -35 to -92. Glucose transporters might play an important role in such isotopic fractionation. Flux analysis showed that both isotopic discrimination and isotopic impurities in labeled substrates could affect the solution of 13C MFA.

KW - Amino acid

KW - Glucose transporter

KW - Isotopic impurity

KW - Mass spectrometer

UR - https://www.scopus.com/pages/publications/80051549277

U2 - 10.1016/j.ab.2011.06.022

DO - 10.1016/j.ab.2011.06.022

M3 - Article

C2 - 21745456

AN - SCOPUS:80051549277

SN - 0003-2697

VL - 417

SP - 295

EP - 297

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Evaluation of isotope discrimination in ¹³C-based metabolic flux analysis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this