TY - JOUR
T1 - Evaluation of a Novel Cryoprobe for Atrial Ablation in a Chronic Ovine Model
AU - Schill, Matthew R.
AU - Melby, Spencer J.
AU - Speltz, Molly
AU - Breitbach, May
AU - Schuessler, Richard B.
AU - Damiano, Ralph J.
PY - 2017/9
Y1 - 2017/9
N2 - Purpose Cryoablation is used in the treatment of atrial fibrillation and other cardiac arrhythmias. This study evaluated a novel 10-cm flexible nitrous oxide cryoprobe in an ovine model of atrial ablation. Description Six sheep were anesthetized, underwent a left thoracotomy, and were placed on cardiopulmonary bypass. A left atriotomy was performed, and the cryoprobe was applied endocardially for 120 seconds at less than –40°C to 4 sites on the left atrium. The atrium was closed and the animals were allowed to recover. After 30 days, the animals were euthanized. Transmurality was evaluated in 5-mm sections of each lesion using 2,3,5-triphenyltetrazolium chloride (TTC) and Masson's trichrome staining. Evaluation All animals survived. One hundred four of 106 sections (98%) were transmural by TTC; 103 of 106 (97%) sections were transmural by trichrome staining. There was no late atrial perforation, intraluminal thrombus, or thromboembolism. Conclusions The device reliably produced transmural lesions in a chronic ovine model. Its performance was equivalent to that of other nitrous oxide cryoablation systems.
AB - Purpose Cryoablation is used in the treatment of atrial fibrillation and other cardiac arrhythmias. This study evaluated a novel 10-cm flexible nitrous oxide cryoprobe in an ovine model of atrial ablation. Description Six sheep were anesthetized, underwent a left thoracotomy, and were placed on cardiopulmonary bypass. A left atriotomy was performed, and the cryoprobe was applied endocardially for 120 seconds at less than –40°C to 4 sites on the left atrium. The atrium was closed and the animals were allowed to recover. After 30 days, the animals were euthanized. Transmurality was evaluated in 5-mm sections of each lesion using 2,3,5-triphenyltetrazolium chloride (TTC) and Masson's trichrome staining. Evaluation All animals survived. One hundred four of 106 sections (98%) were transmural by TTC; 103 of 106 (97%) sections were transmural by trichrome staining. There was no late atrial perforation, intraluminal thrombus, or thromboembolism. Conclusions The device reliably produced transmural lesions in a chronic ovine model. Its performance was equivalent to that of other nitrous oxide cryoablation systems.
UR - http://www.scopus.com/inward/record.url?scp=85028327958&partnerID=8YFLogxK
U2 - 10.1016/j.athoracsur.2017.02.041
DO - 10.1016/j.athoracsur.2017.02.041
M3 - Article
C2 - 28838486
AN - SCOPUS:85028327958
VL - 104
SP - 1069
EP - 1073
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
SN - 0003-4975
IS - 3
ER -