Evaluation of a genetically engineered cell line and a histochemical β- galactosidase assay to detect herpes simplex virus in clinical specimens

E. C. Stabell; S. R. O'Rourke; G. A. Storch; P. D. Olivo

doi:10.1128/jcm.31.10.2796-2798.1993

Evaluation of a genetically engineered cell line and a histochemical β- galactosidase assay to detect herpes simplex virus in clinical specimens

E. C. Stabell, S. R. O'Rourke, G. A. Storch, P. D. Olivo

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Abstract

A novel histochemical method was compared with a cytopathic effect (CPE) assay for the identification of herpes simplex virus (HSV) in clinical specimens. The method utilizes a stably transformed cell line (BHKICP6LacZ- 5) that expresses β-galactosidase only after infection with HSV. A total of 96 specimens submitted to our diagnostic virology laboratory were analyzed. Thirty-one specimens contained HSV as evidenced by positive CPE, and all were positive for β-galactosidase staining. CPE were not evident for 2 or more days in 15 of the 31 positive specimens, whereas the histochemical stain was positive in all 31 positives by 16 to 24 h. This preliminary study shows that the BHKICP6LacZ-5 cell line can be used in a rapid, sensitive, and specific assay for the detection of HSV in clinical specimens.

Original language	English
Pages (from-to)	2796-2798
Number of pages	3
Journal	Journal of clinical microbiology
Volume	31
Issue number	10
DOIs	https://doi.org/10.1128/jcm.31.10.2796-2798.1993
State	Published - 1993

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10.1128/jcm.31.10.2796-2798.1993

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title = "Evaluation of a genetically engineered cell line and a histochemical β- galactosidase assay to detect herpes simplex virus in clinical specimens",

abstract = "A novel histochemical method was compared with a cytopathic effect (CPE) assay for the identification of herpes simplex virus (HSV) in clinical specimens. The method utilizes a stably transformed cell line (BHKICP6LacZ- 5) that expresses β-galactosidase only after infection with HSV. A total of 96 specimens submitted to our diagnostic virology laboratory were analyzed. Thirty-one specimens contained HSV as evidenced by positive CPE, and all were positive for β-galactosidase staining. CPE were not evident for 2 or more days in 15 of the 31 positive specimens, whereas the histochemical stain was positive in all 31 positives by 16 to 24 h. This preliminary study shows that the BHKICP6LacZ-5 cell line can be used in a rapid, sensitive, and specific assay for the detection of HSV in clinical specimens.",

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T1 - Evaluation of a genetically engineered cell line and a histochemical β- galactosidase assay to detect herpes simplex virus in clinical specimens

AU - Stabell, E. C.

AU - O'Rourke, S. R.

AU - Storch, G. A.

AU - Olivo, P. D.

PY - 1993

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N2 - A novel histochemical method was compared with a cytopathic effect (CPE) assay for the identification of herpes simplex virus (HSV) in clinical specimens. The method utilizes a stably transformed cell line (BHKICP6LacZ- 5) that expresses β-galactosidase only after infection with HSV. A total of 96 specimens submitted to our diagnostic virology laboratory were analyzed. Thirty-one specimens contained HSV as evidenced by positive CPE, and all were positive for β-galactosidase staining. CPE were not evident for 2 or more days in 15 of the 31 positive specimens, whereas the histochemical stain was positive in all 31 positives by 16 to 24 h. This preliminary study shows that the BHKICP6LacZ-5 cell line can be used in a rapid, sensitive, and specific assay for the detection of HSV in clinical specimens.

AB - A novel histochemical method was compared with a cytopathic effect (CPE) assay for the identification of herpes simplex virus (HSV) in clinical specimens. The method utilizes a stably transformed cell line (BHKICP6LacZ- 5) that expresses β-galactosidase only after infection with HSV. A total of 96 specimens submitted to our diagnostic virology laboratory were analyzed. Thirty-one specimens contained HSV as evidenced by positive CPE, and all were positive for β-galactosidase staining. CPE were not evident for 2 or more days in 15 of the 31 positive specimens, whereas the histochemical stain was positive in all 31 positives by 16 to 24 h. This preliminary study shows that the BHKICP6LacZ-5 cell line can be used in a rapid, sensitive, and specific assay for the detection of HSV in clinical specimens.

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DO - 10.1128/jcm.31.10.2796-2798.1993

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Evaluation of a genetically engineered cell line and a histochemical β- galactosidase assay to detect herpes simplex virus in clinical specimens

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