Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens

P. P. Young, R. S. Buller, G. A. Storch

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.

Original languageEnglish
Pages (from-to)4260-4261
Number of pages2
JournalJournal of clinical microbiology
Volume38
Issue number11
StatePublished - Dec 1 2000

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