Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens

P. P. Young; R. S. Buller; G. A. Storch

doi:10.1128/jcm.38.11.4260-4261.2000

Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens

P. P. Young, R. S. Buller, G. A. Storch

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.

Original language	English
Pages (from-to)	4260-4261
Number of pages	2
Journal	Journal of clinical microbiology
Volume	38
Issue number	11
DOIs	https://doi.org/10.1128/jcm.38.11.4260-4261.2000
State	Published - 2000

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10.1128/jcm.38.11.4260-4261.2000

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Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens

Abstract

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