Eukaryotic expression systems for structural studies

Christopher A. Nelson; William H. McCoy; Daved H. Fremont

doi:10.1007/978-1-4939-0354-2_8

Eukaryotic expression systems for structural studies

Christopher A. Nelson, William H. McCoy, Daved H. Fremont

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

This protocol describes protein production in mammalian cells by transient transfection. It assumes the expression construct contains either a 6-HIS or Fc fusion tag to allow recovery of the protein by affinity chromatography. The method is one of the simplest available for protein expression in eukaryotic cells, requires little specialized equipment, and has a reasonably high rate of success.

Original language	English
Pages (from-to)	107-116
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1140
DOIs	https://doi.org/10.1007/978-1-4939-0354-2_8
State	Published - 2014

Keywords

CMV expression vector
HEK293-F cells
Kifunensine
Kozak
Mammalian cell
PEI
Polyethylenimine
Signal sequence
Transient transfection

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10.1007/978-1-4939-0354-2_8

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abstract = "This protocol describes protein production in mammalian cells by transient transfection. It assumes the expression construct contains either a 6-HIS or Fc fusion tag to allow recovery of the protein by affinity chromatography. The method is one of the simplest available for protein expression in eukaryotic cells, requires little specialized equipment, and has a reasonably high rate of success.",

keywords = "CMV expression vector, HEK293-F cells, Kifunensine, Kozak, Mammalian cell, PEI, Polyethylenimine, Signal sequence, Transient transfection",

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AU - McCoy, William H.

AU - Fremont, Daved H.

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N2 - This protocol describes protein production in mammalian cells by transient transfection. It assumes the expression construct contains either a 6-HIS or Fc fusion tag to allow recovery of the protein by affinity chromatography. The method is one of the simplest available for protein expression in eukaryotic cells, requires little specialized equipment, and has a reasonably high rate of success.

AB - This protocol describes protein production in mammalian cells by transient transfection. It assumes the expression construct contains either a 6-HIS or Fc fusion tag to allow recovery of the protein by affinity chromatography. The method is one of the simplest available for protein expression in eukaryotic cells, requires little specialized equipment, and has a reasonably high rate of success.

KW - CMV expression vector

KW - HEK293-F cells

KW - Kifunensine

KW - Kozak

KW - Mammalian cell

KW - PEI

KW - Polyethylenimine

KW - Signal sequence

KW - Transient transfection

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Eukaryotic expression systems for structural studies

Abstract

Keywords

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