TY - JOUR
T1 - Eukaryotic DNA Polymerases and δ
T2 - Conserved Properties and Interactions, from Yeast to Mammalian Cells
AU - Burgers, Peter M.J.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - This chapter discusses the DNA polymerases α and δ from mammalian cells and their accessory factors, and on the analogous DNA polymerases from Drosophila melanogaster and Saccharomyces cerevisiae. DNA-polymerizing activity in eukaryotic tissues was discovered not long after Kornberg's discovery of DNA polymerase I from Escherichia coli. The gene for human DNA polymerase α has been isolated and characterized, and new high-fidelity forms of the enzyme have been purified, using rapid isolation techniques. DNA polymerase δ has gained considerable attention because of its interaction with a cell-cycle-regulated protein. This protein, proliferating cell nuclear antigen (PCNA), or cyclin, is also required for in vitro DNA replication of simian-virus-40 (SV40) DNA replication. The major differentiating characteristics of the four mammalian DNA polymerases are summarized in the chapter through a table. DNA polymerases have been isolated from a variety of unicellular eukaryotes. A comparison of the polymerases from these organisms with the prototypical mammalian DNA polymerases is problematic for several reasons that are described in the chapter.
AB - This chapter discusses the DNA polymerases α and δ from mammalian cells and their accessory factors, and on the analogous DNA polymerases from Drosophila melanogaster and Saccharomyces cerevisiae. DNA-polymerizing activity in eukaryotic tissues was discovered not long after Kornberg's discovery of DNA polymerase I from Escherichia coli. The gene for human DNA polymerase α has been isolated and characterized, and new high-fidelity forms of the enzyme have been purified, using rapid isolation techniques. DNA polymerase δ has gained considerable attention because of its interaction with a cell-cycle-regulated protein. This protein, proliferating cell nuclear antigen (PCNA), or cyclin, is also required for in vitro DNA replication of simian-virus-40 (SV40) DNA replication. The major differentiating characteristics of the four mammalian DNA polymerases are summarized in the chapter through a table. DNA polymerases have been isolated from a variety of unicellular eukaryotes. A comparison of the polymerases from these organisms with the prototypical mammalian DNA polymerases is problematic for several reasons that are described in the chapter.
UR - http://www.scopus.com/inward/record.url?scp=0024310831&partnerID=8YFLogxK
U2 - 10.1016/S0079-6603(08)60700-X
DO - 10.1016/S0079-6603(08)60700-X
M3 - Article
C2 - 2505329
AN - SCOPUS:0024310831
SN - 0079-6603
VL - 37
SP - 235
EP - 280
JO - Progress in Nucleic Acid Research and Molecular Biology
JF - Progress in Nucleic Acid Research and Molecular Biology
IS - C
ER -