ETV2/ER71 regulates hematopoietic regeneration by promoting hematopoietic stem cell proliferation

Can Xin Xu, Tae Jin Lee, Nagisa Sakurai, Karen Krchma, Fang Liu, Daofeng Li, Ting Wang, Kyunghee Choi

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Recent studies have established that hematopoietic stem cells (HSCs) are quiescent in homeostatic conditions but undergo extensive cell cycle and expansion upon bone marrow (BM) transplantation or hematopoietic injury. The molecular basis for HSC activation and expansion is not completely understood. In this study, we found that key developmentally critical genes controlling hematopoietic stem and progenitor cell (HSPC) generation were up-regulated in HSPCs upon hematopoietic injury. In particular, we found that the ETS transcription factor Ets variant 2 (Etv2; also known as Er71) was up-regulated by reactive oxygen species in HSPCs and was necessary in a cell-autonomous manner for HSPC expansion and regeneration after BM transplantation and hematopoietic injury. We found c-Kit to be downstream of ETV2. As such, lentiviral c-Kit expression rescued Etv2-deficient HSPC proliferation defects in vitro and in short-term BM transplantation in vivo. These findings demonstrate that Etv2 is an important regulator of hematopoietic regeneration.

Original languageEnglish
Pages (from-to)1643-1653
Number of pages11
JournalJournal of Experimental Medicine
Volume214
Issue number6
DOIs
StatePublished - Jun 1 2017

Fingerprint Dive into the research topics of 'ETV2/ER71 regulates hematopoietic regeneration by promoting hematopoietic stem cell proliferation'. Together they form a unique fingerprint.

  • Cite this