Estimation of circular DNA size using γ-irradiation and pulsed-field gel electrophoresis

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Abstract

A method is described for estimating the size of large circular DNAs found within complex chromosomal DNA preparations. DNAs are treated with low levels of γ-irradiation, sufficient to introduce a single doublestranded break per circle, and the resulting linear DNA is sized by pulsed-field electrophoresis and blot hybridization. The method is fast, reproducible, and very conveniently applied to the agarose-enclosed chromosomal DNA preparations commonly used in pulsed field electrophoresis.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalAnalytical Biochemistry
Volume177
Issue number1
DOIs
StatePublished - Feb 15 1989
Externally publishedYes

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