TY - JOUR
T1 - Establishment of a rat model of benign prostatic hyperplasia complicated with late-onset hypogonadism
AU - Qin, Sheng fei
AU - Xu, Jian
AU - Jian, Xiao ming
AU - Chen, Guang hua
AU - Xiao, Guang An
AU - He, Miao xia
AU - Lai, Henry
AU - Zhou, Tie
N1 - Funding Information:
Supported by the "1255" Project of Changhd Hospital of Second Military Medical University (CH125520302).
Publisher Copyright:
© 2017, Second Military Medical University Press. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Objective To establish and assess a rat model of benign prostatic hyperplasia (BPH) complicated with late-onset hypogonadism (LOH). MethocSs A total of 80 SD rats were randomly divided into 4 groups: control group, BPH complicated withLOH (model) group, BPH group andLOH group, with 20 rats in each group. In the model group, intraperitoneal injection of cyclophosphamide (20 mg/[kg · d]) was continuously administered for 5 days, followed by continuous intraperitoneal injection of testosterone propionate (50 mg/[kg · d]) for 28 days; the rats in the BPH group were treated the same as that in the model group, except with the same volume normal saline instead of cyclophosphamide; in the LOH group with same volume lucca oil instead of testosterone propionate; and in the control group, the rats were treated withsame volumenormal saline instead of cyclophosphamide and lucca oil instead of testosterone propionate. Two days after drug withdraw, serum testosterone was detected andweight loading swimming test, tall suspension test and sexual behavior test were performed. Then prostate weight and pathology were determined after the rats were sacrificed by cervical dislocation. Results The mean prostate indexes of rats in the LOH, model, control and BPH groups were 1. 58 ± 0. 13, 2. 93 ± 0. 19, 2. 33 ± 0. 13 and 3 23 ± 0. 11, respectively; serum testosterone levels were (4. 91 ± 1. 06), (9. 52 ± 1. 02), (12. 59 ± 0. 70) and (19. 69 ± 0. 56) ng/mL, respectively; the tail suspension struggling times were 97. 40 ± 15. 86, 120. 40 ± 14. 06, 223. 83 ± 16. 51, and 235. 29 ± 18. 77, respectively; the weight loading swimming timeswere (74. 27 ± 9. 29), (167. 47 ± 23. 35), (302. 33 ± 30. 10) and (261. 59 ± 35. 13) s, respectively; the times of olfactory sensation were 1. 53 ± 0. 52, 3 07 ± 0. 88, 9. 17 ± 1 30 and 9. 59 ± 1. 12, respectively; themean ride across times were 0. 33 ± 0. 49, 0. 47 ± 0. 52, 2. 11 ± 0. 47 and 2. 29 ± 0. 47, respectively. Statistical analyses showed that there were significant differences among the four groups in mean prostate index, serum testosterone level and tall suspension struggling time (P<0. 05, P<0. 01), in weight loading swimming time and time of olfactory sensation (P<0. 01) except between control group and BPH group, and in mean ride across time (P<0. 01) except between control group and BPH group, andmodel group and LOH group. Conclusion A BPH complicated with LOH rat model was successfully established with intraperitoneal injection of cyclophosphamide and testosterone propionate.
AB - Objective To establish and assess a rat model of benign prostatic hyperplasia (BPH) complicated with late-onset hypogonadism (LOH). MethocSs A total of 80 SD rats were randomly divided into 4 groups: control group, BPH complicated withLOH (model) group, BPH group andLOH group, with 20 rats in each group. In the model group, intraperitoneal injection of cyclophosphamide (20 mg/[kg · d]) was continuously administered for 5 days, followed by continuous intraperitoneal injection of testosterone propionate (50 mg/[kg · d]) for 28 days; the rats in the BPH group were treated the same as that in the model group, except with the same volume normal saline instead of cyclophosphamide; in the LOH group with same volume lucca oil instead of testosterone propionate; and in the control group, the rats were treated withsame volumenormal saline instead of cyclophosphamide and lucca oil instead of testosterone propionate. Two days after drug withdraw, serum testosterone was detected andweight loading swimming test, tall suspension test and sexual behavior test were performed. Then prostate weight and pathology were determined after the rats were sacrificed by cervical dislocation. Results The mean prostate indexes of rats in the LOH, model, control and BPH groups were 1. 58 ± 0. 13, 2. 93 ± 0. 19, 2. 33 ± 0. 13 and 3 23 ± 0. 11, respectively; serum testosterone levels were (4. 91 ± 1. 06), (9. 52 ± 1. 02), (12. 59 ± 0. 70) and (19. 69 ± 0. 56) ng/mL, respectively; the tail suspension struggling times were 97. 40 ± 15. 86, 120. 40 ± 14. 06, 223. 83 ± 16. 51, and 235. 29 ± 18. 77, respectively; the weight loading swimming timeswere (74. 27 ± 9. 29), (167. 47 ± 23. 35), (302. 33 ± 30. 10) and (261. 59 ± 35. 13) s, respectively; the times of olfactory sensation were 1. 53 ± 0. 52, 3 07 ± 0. 88, 9. 17 ± 1 30 and 9. 59 ± 1. 12, respectively; themean ride across times were 0. 33 ± 0. 49, 0. 47 ± 0. 52, 2. 11 ± 0. 47 and 2. 29 ± 0. 47, respectively. Statistical analyses showed that there were significant differences among the four groups in mean prostate index, serum testosterone level and tall suspension struggling time (P<0. 05, P<0. 01), in weight loading swimming time and time of olfactory sensation (P<0. 01) except between control group and BPH group, and in mean ride across time (P<0. 01) except between control group and BPH group, andmodel group and LOH group. Conclusion A BPH complicated with LOH rat model was successfully established with intraperitoneal injection of cyclophosphamide and testosterone propionate.
KW - Animal models
KW - Cyclophosphamide
KW - Late-onset hypogonadism
KW - Prostatic hyperplasia
KW - Testosterone propionate
UR - http://www.scopus.com/inward/record.url?scp=85026747302&partnerID=8YFLogxK
U2 - 10.16781/j.0258-879x.2017.07.0865
DO - 10.16781/j.0258-879x.2017.07.0865
M3 - Article
AN - SCOPUS:85026747302
SN - 0258-879X
VL - 38
SP - 865
EP - 870
JO - Academic Journal of Second Military Medical University
JF - Academic Journal of Second Military Medical University
IS - 7
ER -