TY - JOUR
T1 - Establishment and comparative genomic hybridization analysis of human esophageal carcinomas cell line EC9706
AU - Han, Yaling
AU - Wei, Fang
AU - Xu, Xin
AU - Cai, Yan
AU - Chen, Baosheng
AU - Wang, Jie
AU - Xia, Shuhua
AU - Hu, Hai
AU - Huang, Xiaoping
AU - Han, Yusheng
AU - Wu, Min
AU - Wang, Mingrong
N1 - Funding Information:
The authors gratefully acknowledge the fund support from Key Program of National Natural Science Foundation of China (Grant No. 51336007).
PY - 2002/12
Y1 - 2002/12
N2 - Objective: To establish a long-standing cell line of esophageal squamous cell carcinoma (ESCC) in pursuit of a model for in vitro study of carcinogenesis. Methods: Small tissue blocks taken from resected specimens of esophageal cancer were cultured, and cell line EC9706 was established. The biologic properties of EC9706 were characterized. Comparative genomic hybridization (CGH) was performed on the cell line. Results: The growth curve of EC9706 was detected. The cell generation time was 26 hours. The plate colony forming efficiency is 91.9%, with the capacity of forming clones in soft agar. EC9706 cells show high tumorigenecity as indicated by the rapid regeneration of moderate-poor-differentiated squamous cell carcinomas after injection into nude mice. CGH analysis indicated copy number gains of 1p1, 1q2-4, 2p1,2q1,5p,7p14,7q21,11q1,15q2,20q and losses of 2p2,2q2,3p,4,9p,14,18,Xq. High-level gain of 5p was observed. Conclusion: Established cell line EC9706 can serve as a useful tool for studying the carcinogenesis of ESCC.
AB - Objective: To establish a long-standing cell line of esophageal squamous cell carcinoma (ESCC) in pursuit of a model for in vitro study of carcinogenesis. Methods: Small tissue blocks taken from resected specimens of esophageal cancer were cultured, and cell line EC9706 was established. The biologic properties of EC9706 were characterized. Comparative genomic hybridization (CGH) was performed on the cell line. Results: The growth curve of EC9706 was detected. The cell generation time was 26 hours. The plate colony forming efficiency is 91.9%, with the capacity of forming clones in soft agar. EC9706 cells show high tumorigenecity as indicated by the rapid regeneration of moderate-poor-differentiated squamous cell carcinomas after injection into nude mice. CGH analysis indicated copy number gains of 1p1, 1q2-4, 2p1,2q1,5p,7p14,7q21,11q1,15q2,20q and losses of 2p2,2q2,3p,4,9p,14,18,Xq. High-level gain of 5p was observed. Conclusion: Established cell line EC9706 can serve as a useful tool for studying the carcinogenesis of ESCC.
KW - Cell line
KW - Comparative genomic hybridization
KW - Esophageal squamous cell carcinomas
UR - http://www.scopus.com/inward/record.url?scp=12244275736&partnerID=8YFLogxK
M3 - Article
C2 - 12476413
AN - SCOPUS:12244275736
VL - 19
SP - 455
EP - 457
JO - Chinese Journal of Medical Genetics
JF - Chinese Journal of Medical Genetics
SN - 1003-9406
IS - 6
ER -