@inbook{62df10bd1f834236aece21e479dfb5f6,
title = "Establishing TurboID Proximity Labeling and mAID Conditional Knockdown System in Cryptosporidium parvum",
abstract = "Protein interaction networks and subcellular proteomes can provide key insights into the biological functions of protein complexes. Here, we describe a method for proximity-based labeling using TurboID to define protein interaction networks in Cryptosporidium parvum. In addition, we have developed an auxin-inducible degron (AID) labeling system for conditional protein degradation in C. parvumCryptosporidium speciesC. parvum to facilitate functional studies. This chapter describes the procedures for generating miniTurbo or mAID-TIR1-tagged C. parvumCryptosporidium speciesC. parvum strains and validating the TurboID and mAID systems for target genes.",
keywords = "Conditional knockdown, CRISPR/Cas9, Degron, Mass spectrometry, Proximity labeling",
author = "Rui Xu and Sibley, \{L. David\}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.",
year = "2026",
doi = "10.1007/978-1-0716-4824-7\_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "299--316",
booktitle = "Methods in Molecular Biology",
}