Essential role for fibrillin-2 in zebrafish notochord and vascular morphogenesis

John M. Gansner, Erik C. Madsen, Robert P. Mecham, Jonathan D. Gitlin

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Recent studies demonstrate that lysyl oxidase cuproenzymes are critical for zebrafish notochord formation, but the molecular mechanisms of copper-dependent notochord morphogenesis are incompletely understood. We, therefore, conducted a forward genetic screen for zebrafish mutants that exhibit notochord sensitivity to lysyl oxidase inhibition, yielding a mutant with defects in notochord and vascular morphogenesis, puff daddygw1 (pfdgw1). Meiotic mapping and cloning reveal that the pfdgw1 phenotype results from disruption of the gene encoding the extracellular matrix protein fibrillin-2, and the spatiotemporal expression of fibrillin-2 is consistent with the pfd gw1 phenotype. Furthermore, each aspect of the pfdgw1 phenotype is recapitulated by morpholino knockdown of fibrillin-2. Taken together, the data reveal a genetic interaction between fibrillin-2 and the lysyl oxidases in notochord formation and demonstrate the importance of fibrillin-2 in specific early developmental processes in zebrafish.

Original languageEnglish
Pages (from-to)2844-2861
Number of pages18
JournalDevelopmental Dynamics
Volume237
Issue number10
DOIs
StatePublished - Oct 2008

Keywords

  • Caudal vein
  • Congenital contractural arachnodactyly
  • Copper
  • Fibrillin-2
  • Lysyl oxidase
  • Notochord
  • Zebrafish

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