ESCRT factors restrict mycobacterial growth

Jennifer A. Philips, Maura C. Porto, Hui Wang, Eric J. Rubin, Norbert Perrimon

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Nearly 1.7 billion people are infected with Mycobacterium tuberculosis. Its ability to survive intracellularly is thought to be central to its success as a pathogen, but how it does this is poorly understood. Using a Drosophila model of infection, we identify three host cell activities, Rab7, CG8743, and the ESCRT machinery, that modulate the mycobacterial phagosome. In the absence of these factors the cell no longer restricts growth of the nonpathogen Mycobacterium smegmatis. Hence, we identify factors that represent unique vulnerabilities of the host cell, because manipulation of any one of them alone is sufficient to allow a nonpathogenic mycobacterial species to proliferate. Furthermore, we demonstrate that, in mammalian cells, the ESCRT machinery plays a conserved role in restricting bacterial growth.

Original languageEnglish
Pages (from-to)3070-3075
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number8
DOIs
StatePublished - Feb 26 2008

Keywords

  • Mycobacterium
  • Phagosome
  • Tuberculosis
  • Ubiquitin

Fingerprint

Dive into the research topics of 'ESCRT factors restrict mycobacterial growth'. Together they form a unique fingerprint.

Cite this