Escherichia coli O157:H7 in retail ground beef in Seattle: Results of a one-year prospective study

Phillip I. Tarr, Nhiem Thanh Tran, Richard A. Wilson

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Escherichia coli O157:H7 was sought systematically in 1,400 samples of retail ground beef in Seattle in a 1-year prospective study. Sorbitol-nonfermenting, lactose-fermenting, indole-positive colonies isolated after enrichment culture were probed for the presence of Shiga toxin genes. Totals of 67,040 sorbitol-nonfermenting and 66,705 sorbitol-fermenting colonies were characterized, but E. coli O157:H7 was not identified. The sensitivity of this technique was usually sufficient to detect E. coli O157:H7 at a concentration below 1 CFU/g of meat. These data demonstrate that retail ground beef in Seattle is neither frequently not heavily contaminated with E. coli O157:H7.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalJournal of Food Protection
Volume62
Issue number2
DOIs
StatePublished - Feb 1999

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