TY - JOUR
T1 - Escherichia coli O157:H7 in retail ground beef in Seattle
T2 - Results of a one-year prospective study
AU - Tarr, Phillip I.
AU - Tran, Nhiem Thanh
AU - Wilson, Richard A.
PY - 1999/2
Y1 - 1999/2
N2 - Escherichia coli O157:H7 was sought systematically in 1,400 samples of retail ground beef in Seattle in a 1-year prospective study. Sorbitol-nonfermenting, lactose-fermenting, indole-positive colonies isolated after enrichment culture were probed for the presence of Shiga toxin genes. Totals of 67,040 sorbitol-nonfermenting and 66,705 sorbitol-fermenting colonies were characterized, but E. coli O157:H7 was not identified. The sensitivity of this technique was usually sufficient to detect E. coli O157:H7 at a concentration below 1 CFU/g of meat. These data demonstrate that retail ground beef in Seattle is neither frequently not heavily contaminated with E. coli O157:H7.
AB - Escherichia coli O157:H7 was sought systematically in 1,400 samples of retail ground beef in Seattle in a 1-year prospective study. Sorbitol-nonfermenting, lactose-fermenting, indole-positive colonies isolated after enrichment culture were probed for the presence of Shiga toxin genes. Totals of 67,040 sorbitol-nonfermenting and 66,705 sorbitol-fermenting colonies were characterized, but E. coli O157:H7 was not identified. The sensitivity of this technique was usually sufficient to detect E. coli O157:H7 at a concentration below 1 CFU/g of meat. These data demonstrate that retail ground beef in Seattle is neither frequently not heavily contaminated with E. coli O157:H7.
UR - http://www.scopus.com/inward/record.url?scp=0344938000&partnerID=8YFLogxK
U2 - 10.4315/0362-028X-62.2.133
DO - 10.4315/0362-028X-62.2.133
M3 - Article
C2 - 10030631
AN - SCOPUS:0344938000
VL - 62
SP - 133
EP - 139
JO - Journal of Food Protection
JF - Journal of Food Protection
SN - 0362-028X
IS - 2
ER -