TY - JOUR
T1 - Escherichia coli-mediated impairment of ureteric contractility is uropathogenic E. coli specific
AU - Floyd, Rachel V.
AU - Upton, Mathew
AU - Hultgren, Scott J.
AU - Wray, Susan
AU - Burdyga, Theodor V.
AU - Winstanley, Craig
N1 - Funding Information:
Financial support. This work was supported by the Wellcome Trust (grant 094705 to R. V. F., M. U., S. W., T. V. B., and C. W.), Mersey Kidney Research (grant 25/08 to R. V. F., S. W., T. V. B., and C.W.), and the Office of Research on Women’s Health Specialized Center of Research (grants DK64540 and R01-DK079798 to S. J. H.). Potential conflicts of interest. All authors: No reported conflicts.
PY - 2012/11/15
Y1 - 2012/11/15
N2 - Background. Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.Methods.Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.Results.Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8, compared with 8.8 in controls. UPEC effects on contractility were strain specific, with decreases from 9.47 to 96.7. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.Conclusions.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.
AB - Background. Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.Methods.Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.Results.Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8, compared with 8.8 in controls. UPEC effects on contractility were strain specific, with decreases from 9.47 to 96.7. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.Conclusions.We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.
UR - https://www.scopus.com/pages/publications/84867730641
U2 - 10.1093/infdis/jis554
DO - 10.1093/infdis/jis554
M3 - Article
C2 - 23002447
AN - SCOPUS:84867730641
SN - 0022-1899
VL - 206
SP - 1589
EP - 1596
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 10
ER -