Erufosine simultaneously induces apoptosis and autophagy by modulating the Akt-mTOR signaling pathway in oral squamous cell carcinoma

Vaishali Kapoor, Maya M. Zaharieva, Satya N. Das, Martin R. Berger

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

We investigated the anticancer activity of erufosine in oral squamous carcinoma cell lines in terms of cell proliferation, colony formation, induction of autophagy/apoptosis, cell cycle and mTOR signaling pathway. Erufosine showed dose-dependent cytotoxicity in all cell lines, it induced autophagy as well as apoptosis, G2 cell cycle arrest and modulation of cyclin D1 expression. Further erufosine downregulated the phosphorylation of major components of mTOR pathway, like p-Akt at Ser473 and Thr308 residues, p-Raptor, p-mTOR, p-PRAS40 and its downstream substrates p-p70S6K and p-4EBP1 in a dose-dependent manner. The pre-treatment of tumor cells with p-mTOR siRNA increased cytotoxic effects of erufosine comparable to cisplatin but higher than rapamycin.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalCancer Letters
Volume319
Issue number1
DOIs
StatePublished - Jun 1 2012

Keywords

  • Alkylphosphocholine
  • Autophagy
  • Erufosine
  • MTOR signaling cascade
  • Oral squamous cell cancer

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