ER stress inhibits mTORC2 and Akt signaling through GSK-3β-mediated phosphorylation of rictor

Chien Hung Chen, Tattym Shaikenov, Timothy R. Peterson, Rakhan Aimbetov, Amangeldy K. Bissenbaev, Szu Wei Lee, Juan Wu, Hui Kuan Lin, Dos D. Sarbassov

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87 Scopus citations

Abstract

In response to environmental cues, cells coordinate a balance between anabolic and catabolic pathways. In eukaryotes, growth factors promote anabolic processes and stimulate cell growth, proliferation, and survival through activation of the phosphoinositide 3-kinase (PI3K)-Akt pathway. Akt-mediated phosphorylation of glycogen synthase kinase-3β (GSK-3β) inhibits its enzymatic activity, thereby stimulating glycogen synthesis. We show that GSK-3b itself inhibits Akt by controlling the mammalian target of rapamycin complex 2 (mTORC2), a key activating kinase for Akt. We found that during cellular stress, GSK-3β phosphorylated the mTORC2 component rictor at serine-1235, a modification that interfered with the binding of Akt to mTORC2. The inhibitory effect of GSK-3β on mTORC2-Akt signaling and cell proliferation was eliminated by blocking phosphorylation of rictor at serine-1235. Thus, in response to cellular stress, GSK-3β restrains mTORC2-Akt signaling by specifically phosphorylating rictor, thereby balancing the activities of GSK-3β and Akt, two opposing players in glucose metabolism.

Original languageEnglish
Article numberra10
JournalScience signaling
Volume4
Issue number161
DOIs
StatePublished - Feb 22 2011
Externally publishedYes

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    Chen, C. H., Shaikenov, T., Peterson, T. R., Aimbetov, R., Bissenbaev, A. K., Lee, S. W., Wu, J., Lin, H. K., & Sarbassov, D. D. (2011). ER stress inhibits mTORC2 and Akt signaling through GSK-3β-mediated phosphorylation of rictor. Science signaling, 4(161), [ra10]. https://doi.org/10.1126/scisignal.2001731